Escherichia coli and Klebsiella pneumoniae isolates presenting with the same antimicrobial susceptibility profile were recovered from the same catheter sample of urine (CSU). Both strains were recovered from a patient with a long‐standing indwelling urinary catheter. Each isolate had its DNA extracted following culture. Nanopore long‐read sequencing was used to build the plasmids and chromosomes from each strain to closure to discern the potential horizontal propagation of resistance‐encoding plasmids and the relationship between resistance genes and insertion sequences. Plasmids derived from resistance strains in the urinary microbiota remain poorly characterized. The same 11 antimicrobial resistance (AMR) genes were found in plasmids from each strain. The 185,239‐bp FIB(K) pKBM1, from the K. pneumoniae strain, additionally encoded the five AMR genes: sul2, strA, strB, bla TEM‐1B, and bla CTX‐M‐15. A multimeric array of AMR genes and IS 26 insertion sequences were found in the plasmids from both isolates. Both plasmids from each isolate were similar. Horizontal transfer of plasmids, followed by subsequent plasmid rearrangement, is likely to have occurred during infection. Furthermore, the resistance region in the plasmids shared similarity against the internationally prevalent plasmid, pKPN3‐307_typeA, commonly identified in K. pneumoniae ST307. Biofilm formation in catheterized patients may allow close cell contact between strains. Horizontal propagation of resistance genes may occur, leading to polymicrobial infections.
Two bacterial strains were recovered from the same catheter specimen of urine sample. The Escherichia coli strain encoded 11 resistance genes on a plasmid, including the ESBL gene, bla CTX‐M‐15. The Klebsiella pneumoniae strain encoded an additional 5 resistance genes on a plasmid, including 2 copies of bla CTX‐M‐15. Resistance genes were flanked between IS 26 in a multimeric array. Both plasmids were similar, suggesting horizontal transfer occurred during the course of infection.