A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC 50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.
Demonstrated chip platform for HTS of protein expression and toxicity of 3D cultures
Dose-response viability and proliferation of a 24-compound library on human NPC lines
Assessed differential toxicity between progenitors and differentiating progeny
Identified five compounds more toxic to undifferentiated progenitors
Dordick and colleagues demonstrate a 3D cell culture chip for high-throughput screening of immortalized human neural progenitor cells. The dose-response toxicity of 24 compounds was determined on undifferentiated and differentiating hNPCs. Five compounds led to significant differences in IC 50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.