There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

A polyclonal antibody (anti-PepT1/C) was raised against the rabbit intestinal H(+)-coupled oligopeptide transporter, PepT1. Anti-PepT1/C detected 70-80-kDa protein in crude membranes obtained from rabbit duodenum, jejunum and ileum. PepT1 was localized in the brush-border of the absorptive epithelial cells by subcellular fractionation of membranes on a sucrose density gradient and by immunohistochemistry using light and electron microscopy. Transport activity for cephalosporins and dipeptide expressed in Xenopus laevis oocytes injected with total mRNA obtained from rabbit small intestine was eliminated completely by prehybridization of the mRNA with antisense oligonucleotide against the 5'-coding region of rabbit PepT1 cDNA.

Related collections

Author and article information

Journal

PubMed ID:: 8769308

DOI:: 10.1016/0014-5793(96)00778-8

ScienceOpen disciplines: Chemistry

Keywords: Amino Acid Sequence,Animals,Anti-Bacterial Agents,metabolism,pharmacokinetics,Carrier Proteins,Duodenum,Epithelium,Ileum,Immunoblotting,Intestinal Absorption,Intestine, Small,ultrastructure,Jejunum,Microscopy, Immunoelectron,Microvilli,Molecular Sequence Data,Oligonucleotides, Antisense,Rabbits,Symporters,Xenopus laevis,beta-Lactams

Data availability:

ScienceOpen disciplines: Chemistry

Keywords: Amino Acid Sequence, Animals, Anti-Bacterial Agents, metabolism, pharmacokinetics, Carrier Proteins, Duodenum, Epithelium, Ileum, Immunoblotting, Intestinal Absorption, Intestine, Small, ultrastructure, Jejunum, Microscopy, Immunoelectron, Microvilli, Molecular Sequence Data, Oligonucleotides, Antisense, Rabbits, Symporters, Xenopus laevis, beta-Lactams

Comments

Comment on this article

scite_

0

Smart Citations

0

Citing PublicationsSupportingMentioningContrasting

View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

Cited by 4

See all cited by

Immunolocalization and pharmacological relevance of oligopeptide transporter PepT1 in intestinal absorption of beta-lactam antibiotics.

Read this article at