Stability of isoenzyme and kinetoplast DNA (k-DNA) patterns in successively cloned Trypanosoma cruzi populations – ScienceOpen
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      Stability of isoenzyme and kinetoplast DNA (k-DNA) patterns in successively cloned Trypanosoma cruzi populations

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          Abstract

          Four Trypanosoma cruzi strains from zymodermes A, B, C and D were successively clonedon BHI-LIT-agar-blood BLAB). Twenty clones from the first generation (F1), 10 from The second (F2) and 4 from the third (F3) from the strains A138, B147 and C23 were isolated. The D150 strain provied 29 F1 and F2 clones. The strains and clones had their isoenzyme and K-DNA patterns determined. The clones from A138, Bl47 and C231 strains presented isoemzyme and K-DNA patterns identical between thewmselves and their respective parental strains. Therefore showing the homogenety and stability of isoenzyme and K-DNA patterns after successive cloning. The Dl50 strain from zymodeme D (ZD) showed heterogeneity. Twenty-eight out of 29 clones of the first generation were of zymodeme A and only one was of zymodeme C, confirming previous reports that ZD strains consisted of ZA and ZC parasite populations. The only D150 strain clone of zymodeme C showed a K-DNA pattern identical to its parental strain. The remining clones although similar among themselves were different from the parental strain. Thus the T. cruzi strains had either homonogeneus or heterogeneous populations. The clones produced by successive cloning provided genetically homonogeous populations. Their experimental use will make future results more reliable and reproducible.

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          Author and article information

          Journal
          mioc
          Memórias do Instituto Oswaldo Cruz
          Mem. Inst. Oswaldo Cruz
          Instituto Oswaldo Cruz, Ministério da Saúde (Rio de Janeiro, RJ, Brazil )
          0074-0276
          1678-8060
          December 1991
          : 86
          : 4
          : 379-385
          Affiliations
          [01] Maringá orgnameUniversidade Estadual de Maringá orgdiv1Depto de Análises Clínicas Brasil
          [02] Belo Horizonte orgnameFIOCRUZ orgdiv1Centro de Pesquisas René Rachou Brasil
          [03] Rio de Janeiro orgnameInstituto Oswaldo Cruz orgdiv1Depto de Bioquímica e Biologia Molecular Brasil
          [04] Belo Horizonte orgnameUFMG orgdiv1ICB orgdiv2Depto de Parasitologia Brasil
          Article
          S0074-02761991000400001 S0074-0276(91)08600401
          10.1590/S0074-02761991000400001
          0a19abc3-d6ba-4c64-b658-2bbde85e72f8

          This work is licensed under a Creative Commons Attribution 4.0 International License.

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          Figures: 0, Tables: 0, Equations: 0, References: 0, Pages: 7
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          SciELO Brazil


          isoenzyme,clones,Trypanosoma cruzi,kenetoplast DNA
          isoenzyme, clones, Trypanosoma cruzi, kenetoplast DNA

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