2
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Stability of isoenzyme and kinetoplast DNA (k-DNA) patterns in successively cloned Trypanosoma cruzi populations

      research-article

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Four Trypanosoma cruzi strains from zymodermes A, B, C and D were successively clonedon BHI-LIT-agar-blood BLAB). Twenty clones from the first generation (F1), 10 from The second (F2) and 4 from the third (F3) from the strains A138, B147 and C23 were isolated. The D150 strain provied 29 F1 and F2 clones. The strains and clones had their isoenzyme and K-DNA patterns determined. The clones from A138, Bl47 and C231 strains presented isoemzyme and K-DNA patterns identical between thewmselves and their respective parental strains. Therefore showing the homogenety and stability of isoenzyme and K-DNA patterns after successive cloning. The Dl50 strain from zymodeme D (ZD) showed heterogeneity. Twenty-eight out of 29 clones of the first generation were of zymodeme A and only one was of zymodeme C, confirming previous reports that ZD strains consisted of ZA and ZC parasite populations. The only D150 strain clone of zymodeme C showed a K-DNA pattern identical to its parental strain. The remining clones although similar among themselves were different from the parental strain. Thus the T. cruzi strains had either homonogeneus or heterogeneous populations. The clones produced by successive cloning provided genetically homonogeous populations. Their experimental use will make future results more reliable and reproducible.

          Related collections

          Author and article information

          Contributors
          Role: ND
          Role: ND
          Role: ND
          Role: ND
          Journal
          mioc
          Memórias do Instituto Oswaldo Cruz
          Mem. Inst. Oswaldo Cruz
          Instituto Oswaldo Cruz, Ministério da Saúde (Rio de Janeiro )
          1678-8060
          December 1991
          : 86
          : 4
          : 379-385
          Affiliations
          [1 ] Universidade Estadual de Maringá Brazil
          [2 ] FIOCRUZ Brasil
          [3 ] Instituto Oswaldo Cruz Brasil
          [4 ] UFMG Brasil
          Article
          S0074-02761991000400001
          10.1590/S0074-02761991000400001
          0a19abc3-d6ba-4c64-b658-2bbde85e72f8

          http://creativecommons.org/licenses/by/4.0/

          History
          Product

          SciELO Brazil

          Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0074-0276&lng=en
          Categories
          PARASITOLOGY
          TROPICAL MEDICINE

          Parasitology,Infectious disease & Microbiology
          isoenzyme,kenetoplast DNA,Trypanosoma cruzi,clones
          Parasitology, Infectious disease & Microbiology
          isoenzyme, kenetoplast DNA, Trypanosoma cruzi, clones

          Comments

          Comment on this article

          scite_
          0
          0
          0
          0
          Smart Citations
          0
          0
          0
          0
          Citing PublicationsSupportingMentioningContrasting
          View Citations

          See how this article has been cited at scite.ai

          scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.

          Similar content187

          Cited by6