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      Phialolunulospora vermispora (Chaetosphaeriaceae, Sordariomycetes), a novel asexual genus and species from freshwater in southern China

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          Abstract

          The asexual taxon Phialolunulospora vermispora gen. et sp. nov., collected from submerged dicotyledonous leaves in Hainan, China, is described and illustrated herein. Phialolunulospora gen. nov. is characterized by macronematous, semimacronematous, septate and pigmented conidiophores and acrogenous, long lunate, vermiform to sigmoid, hyaline conidia with an eccentric basal appendage. Complete sequences of internal transcribed spacer (ITS) and partial sequences of nuclear large subunits ribosomal DNA (LSU) genes are provided. Phylogenetic analyses of combined ITS and LSU sequences revealed its placement in the Chaetosphaeriaceae . The new fungus is compared with morphologically similar genera.

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          Basic local alignment search tool.

          Stephen F Altschul, Warren Gish, Webb Miller … (1990)
          A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score. Recent mathematical results on the stochastic properties of MSP scores allow an analysis of the performance of this method as well as the statistical significance of alignments it generates. The basic algorithm is simple and robust; it can be implemented in a number of ways and applied in a variety of contexts including straightforward DNA and protein sequence database searches, motif searches, gene identification searches, and in the analysis of multiple regions of similarity in long DNA sequences. In addition to its flexibility and tractability to mathematical analysis, BLAST is an order of magnitude faster than existing sequence comparison tools of comparable sensitivity.
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            MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

            Koichiro Tamura, Glen Stecher, Daniel Walter Peterson … (2013)
            We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference by providing a graphical user interface (GUI) to specify the phylogeny and calibration constraints step-by-step. This version also contains enhanced algorithms to search for the optimal trees under evolutionary criteria and implements a more advanced memory management that can double the size of sequence data sets to which MEGA can be applied. Both GUI and command-line versions of MEGA6 can be downloaded from www.megasoftware.net free of charge.
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              AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS

              T.J White, T. Bruns, S. Lee … (1990)
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                Author and article information

                Contributors
                Zefen Yu
                Journal
                Journal ID (nlm-ta): MycoKeys
                Journal ID (iso-abbrev): MycoKeys
                Journal ID (publisher-id): 11
                Journal ID (index): urn:lsid:arphahub.com:pub:C004A564-9D6A-5F9F-B058-6A3815DFE9C3
                Title: MycoKeys
                Publisher: Pensoft Publishers
                ISSN (Print): 1314-4057
                ISSN (Electronic): 1314-4049
                Publication date Collection: 2020
                Publication date (Electronic): 22 December 2020
                Volume: 76
                Pages: 17-30
                Affiliations
                [1 ] Laboratory for Conservation and Utilization of Bio-resources, Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, China Yunnan University Kunming China
                [2 ] Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt” (INIFAT), 17200, La Habana, Cuba Instituto de Investigaciones Fundamentales en Agricultura Tropical “Alejandro de Humboldt” La Habana Cuba
                Author notes
                Corresponding author: Zefen Yu ( zfyuqm@ 123456hotmail.com )

                Academic editor: H. Raja

                Author information
                https://orcid.org/0000-0003-0063-3265
                Article
                Publisher ID: 57410
                DOI: 10.3897/mycokeys.76.57410
                PMC ID: 7772286
                PubMed ID: 33384571
                SO-VID: 09b2d405-e3e0-42d8-ae63-2dbc941fe6a0
                Copyright © Hua Zheng, Yake Wan, Jie Li, Rafael F. Castañeda-Ruiz, Zefen Yu
                License:

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 09 August 2020
                Date accepted : 06 December 2020
                Funding
                Funded by: National Natural Science Foundation of China 501100001809 http://doi.org/10.13039/501100001809
                Categories
                Subject: Research Article
                Subject: Anamorphic Fungi
                Subject: Ascomycota
                Subject: Chaetosphaeriaceae
                Subject: Chaetosphaeriales
                Subject: Sordariomycetes
                Subject: Habitats
                Subject: Ecosystems & Natural Spaces
                Subject: Molecular Systematics
                Subject: Phylogeny
                Subject: Taxonomy
                Subject: Asia
                Subject: Middle East

                Keywords: biodiversity, chaetosphaeriales ,phylogeny,taxonomy
                Data availability:
                Keywords: biodiversity, chaetosphaeriales , phylogeny, taxonomy

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