Estimación del tamaño del genoma en especies de la tribu Cestreae (Solanaceae) mediante citometría de imagen

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Abstract

Los géneros Cestrum, Sessea y Vestia pertenecen a la tribu Cestreae y presentan varias características cromosómicas que los diferencian de otras especies de Solanaceae, como el número y tamaño cromosómico. El tamaño del genoma contribuiría a generar nuevas hipótesis sobre su evolución en los clados basales de Solanaceae. Para estimar el tamaño del genoma en especies de Cestreae fue utilizada la citometría de imagen de núcleos en interfase y telofase teñidos con la técnica de Feulgen. Los valores del tamaño del genoma en las especies analizadas de Cestreae varían entre 17,22 a 24,40 Gpb (2C), siendo los mayores tamaños de genoma de la familia. Además los resultados demuestran que la citometría de imagen es un método alternativo a la citometría de flujo que permite la determinación del tamaño del genoma en forma práctica y económica.

Translated abstract

Cestrum, Sessea and Vestia genera belong to Cestreae tribe and have several chromosome characteristics that differentiate it from other species of Solanaceae, such as number and size. Genome size would contribute to new hypotheses about evolution in basal clades of Solanaceae. To estimate the genome size Cestreae species was used image cytometry of telophase and interphase nuclei stained with Feulgen technique. The values of genome size in species analyzed ranging from 17.22 to 24.40 Gpb (2C), being the largest genome sizes of the family. Furthermore, the results show that the image cytometry is an alternative method to flow cytometry for the determination of the genome size in practical and economical way.

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Most cited references 53

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Nuclear dna amounts in angiosperms.

Derrick Bennett, J. G. Smith (1976)

The number of angiosperm species for which nuclear DNA amount estimates have been made has nearly trebled since the last collected lists of such values were published, and therefore, publication of a more comprehensive list is over due. This paper lists absolute nuclear DNA amounts for 753 angiosperm species. The dats were assembled primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users. The paper also reviews aspects of the history, nomenclature, methods, accuracy and problems of nuclear DNA estimation in angiosperms. No attempt is made to reconsider those aspects of nuclear DNA estimation which have been fully revised previously, although the bibliography of such aspects is given. Instead, the paper is intended as a source of basic information regarding the terminology, practice and limitations of nuclear DNA estimation, especially by Feulgen microdensitometry, as currently practiced.