Journal ID (nlm-ta): PLoS One
Journal ID (iso-abbrev): PLoS ONE
Journal ID (publisher-id): plos
Journal ID (pmc): plosone
Title:
PLoS ONE
Publisher:
Public Library of Science
(San Francisco, CA USA
)
ISSN
(Electronic):
1932-6203
Publication date
(Electronic):
2
June
2017
Publication date Collection: 2017
Volume: 12
Issue: 6
Electronic Location Identifier: e0177788
Affiliations
[1
]Center for Vaccines and Immunity, The Research Institute at Nationwide Children’s
Hospital, Columbus, Ohio, United States of America
[2
]First Biotech Inc., Athens, Ohio, United States of America
[3
]Heidelberg College, Tiffin, Ohio, United States of America
[4
]Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital,
Columbus, Ohio, United States of America
[5
]Department of Pediatrics, The Ohio State University College of Medicine, Columbus,
Ohio, United States of America
[6
]Integrated Biomedical Sciences Graduate Program, The Ohio State University College
of Medicine, Columbus, Ohio, United States of America
University of Helsinki, FINLAND
Author notes
Competing Interests: LKH is affiliated with First Biotech Inc as an employee. LKH and MEP are named as
inventors in a US Patent on Unrestricted mutagenesis and cloning methods (US patent
No. US20110256540 A1. This does not alter our adherence to PLOS ONE policies on sharing
data and materials. The URMAC technology is owned by First Biotech Inc., Columbus,
OH. For licensing inquiries please contact
lhallak@
123456firstbiotech.com
.
-
-
Investigation: LKH KB RK AK.
-
Methodology: LKH MEP AK FM.
-
Writing – original draft: LKH MEP AK FM.
-
Writing – review & editing: LKH MEP FM.
[¤a]
Current address: Genetic Counseling Program, College of Medicine, University of Cincinnati
and Division of Human Genetics, Cincinnati Children’s Hospital Medical Center, Cincinnati,
Ohio, United States of America
[¤b]
Current address: Gene Therapy Branch, FDA/CBER/OCTGT/DCGT, Silver Spring, Maryland,
United States of America
[¤c]
Current address: The Institute of Child Health, University College London, Dubowitz
Neuromuscular Centre, London, United Kingdom
Author information
Article
Publisher ID:
PONE-D-16-51638
DOI: 10.1371/journal.pone.0177788
PMC ID: 5456045
PubMed ID: 28575024
SO-VID: 006b71c1-6ddd-4a88-a589-1ace714f444a
Copyright © © 2017 Hallak et al
License:
This is an open access article distributed under the terms of the
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided
the original author and source are credited.
The URMAC concept, validation, and development including mutagenesis of pUC18 were
supported by L.K.H. personal resources. Mutagenesis of pCG-H, pCG-F, RSV replicon,
and related research were supported by a National Institutes of Health grant number
HL051818 to M.E.P., and by The Research Institute at Nationwide Children’s Hospital.
Mutagenesis of the dystrophin cDNA and its related research were done in the F.M.
laboratory and supported by the Research Institute at Nationwide Children’s Hospital.
R.K. was supported by a T32 grant from the NIH Roadmap Training Program in Clinical
Research.
Subject:
Research Article
Subject:
Biology and Life Sciences
Subject:
Molecular Biology
Subject:
Molecular Biology Techniques
Subject:
Artificial Gene Amplification and Extension
Subject:
Polymerase Chain Reaction
Subject:
Research and Analysis Methods
Subject:
Molecular Biology Techniques
Subject:
Artificial Gene Amplification and Extension
Subject:
Polymerase Chain Reaction
Subject:
Biology and Life Sciences
Subject:
Genetics
Subject:
Mutagenesis
Subject:
Biology and life sciences
Subject:
Molecular biology
Subject:
Molecular biology techniques
Subject:
DNA construction
Subject:
Plasmid Construction
Subject:
Research and analysis methods
Subject:
Molecular biology techniques
Subject:
DNA construction
Subject:
Plasmid Construction
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
DNA
Subject:
Forms of DNA
Subject:
Circular DNA
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
DNA
Subject:
Forms of DNA
Subject:
Circular DNA
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
DNA
Subject:
DNA modification
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
DNA
Subject:
DNA modification
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
Epigenetics
Subject:
DNA modification
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
Gene expression
Subject:
DNA modification
Subject:
Research and Analysis Methods
Subject:
Database and Informatics Methods
Subject:
Bioinformatics
Subject:
Sequence Analysis
Subject:
Sequence Motif Analysis
Subject:
Biology and Life Sciences
Subject:
Genetics
Subject:
Mutation
Subject:
Substitution Mutation
Subject:
Biology and life sciences
Subject:
Genetics
Subject:
DNA
Subject:
Forms of DNA
Subject:
Complementary DNA
Subject:
Biology and life sciences
Subject:
Biochemistry
Subject:
Nucleic acids
Subject:
DNA
Subject:
Forms of DNA
Subject:
Complementary DNA