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      Cytolethal distending toxin in Escherichia coli O157:H7: spectrum of conservation, structure, and endothelial toxicity.

      Journal of Clinical Microbiology
      Alleles, Bacterial Toxins, chemistry, genetics, toxicity, Base Sequence, Cell Line, Conserved Sequence, DNA, Bacterial, Diarrhea, microbiology, Endothelium, Vascular, drug effects, Escherichia coli Infections, Escherichia coli O157, pathogenicity, Evolution, Molecular, Genes, Bacterial, Hemolytic-Uremic Syndrome, Humans, Multigene Family, Virulence

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          Abstract

          We identified the cytolethal distending toxin V (CDT-V) gene cluster in 19 (4.9%) of 391 enterohemorrhagic Escherichia coli O157:H7. cdt-V+ strains belonged to five phage types (PTs) and were most frequent within PTs 14 and 34. CDT-V was expressed in all but two cdt-V+ strains and was lethal to cultured endothelial cells. Subtyping schemes should include cdt-V as a marker to differentiate E. coli O157:H7 even within the same phage type.

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