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      Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR.

      Journal of Clinical Microbiology
      Amino Acid Substitution, genetics, Antiviral Agents, pharmacology, Drug Resistance, Viral, Humans, Influenza A Virus, H1N1 Subtype, drug effects, isolation & purification, Influenza, Human, virology, Molecular Sequence Data, Mutation, Missense, Neuraminidase, Oseltamivir, RNA, Viral, Reverse Transcriptase Polymerase Chain Reaction, methods, Sequence Analysis, DNA, Time Factors, Viral Proteins, Virology

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          Abstract

          The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

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