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      Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.

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      Journal: Antimicrobial Agents and Chemotherapy
      Keywords: Anti-Bacterial Agents, pharmacology, Bacterial Proteins, genetics, Carbapenems, Drug Resistance, Multiple, Bacterial, Klebsiella pneumoniae, drug effects, enzymology, Real-Time Polymerase Chain Reaction, methods, beta-Lactamases

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          Abstract

          We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

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          Journal
          PubMed ID:: 22450983
          PMC ID:: 3370784
          DOI:: 10.1128/AAC.00316-12

          ScienceOpen disciplines: Chemistry
          Keywords: Anti-Bacterial Agents,pharmacology,Bacterial Proteins,genetics,Carbapenems,Drug Resistance, Multiple, Bacterial,Klebsiella pneumoniae,drug effects,enzymology,Real-Time Polymerase Chain Reaction,methods,beta-Lactamases
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          ScienceOpen disciplines: Chemistry
          Keywords: Anti-Bacterial Agents, pharmacology, Bacterial Proteins, genetics, Carbapenems, Drug Resistance, Multiple, Bacterial, Klebsiella pneumoniae, drug effects, enzymology, Real-Time Polymerase Chain Reaction, methods, beta-Lactamases

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