Recommendations for the generation, quantification, storage and handling of peptides used for mass spectrometry-based assays

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Abstract

For many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotope-labeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays.

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Author and article information

Journal

Journal ID (nlm-journal-id): 9421549

Journal ID (pubmed-jr-id): 2992

Journal ID (nlm-ta): Clin Chem

Journal ID (iso-abbrev): Clin. Chem.

Title: Clinical chemistry

ISSN (Print): 0009-9147

ISSN (Electronic): 1530-8561

Publication date Nihms-submitted: 18 March 2016

Publication date (Print): January 2016

Publication date PMC-release: 01 January 2017

Volume: 62

Issue: 1

Pages: 48-69

Affiliations

[1 ]University of Washington, Seattle, WA

[2 ]Fred Hutchinson Cancer Research Center, Seattle, WA

[3 ]Broad Institute, Cambridge, MA

[4 ]Pacific Northwest National Laboratory, Richland, WA

[5 ]New England Peptide, Inc., Gardner, MA

[6 ]Johns Hopkins University, Baltimore, MD

[7 ]Washington University, St Louis, MO

[8 ]Vanderbilt University, Nashville, TN

[9 ]National Cancer Institute, Bethesda, MD

[10 ]ESAC, Inc., Rockville, MD

[11 ]Frederick National Lab for Cancer Research, Frederick, MD

[12 ]University of North Carolina School of Medicine, Chapel Hill, NC

[13 ]Eli Lilly and Company, Indianapolis, IN

[14 ]Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland

[15 ]Mayo Clinic College of Medicine, Rochester, MN

[16 ]National Institute of Standards and Technology, Gaithersburg, MD

[17 ]Quest Diagnostics, San Juan Capistrano, CA

[18 ]21st Century Biochemicals, Inc., Marlborough, MA

[19 ]Laboratory Corporation of America Holdings, Inc., Burlington, NC

[20 ]Institute for Systems Biology, Seattle, WA

[21 ]University of Utah and ARUP Laboratories, Salt Lake City, UT

[22 ]Pfizer, Inc., Andover, MA

[23 ]Gilead Sciences, Inc., Foster City, CA

[24 ]Thermo Fisher Scientific, Rockford, IL

[25 ]Cedars Sinai Medical Center, Los Angeles, CA

[26 ]Wake Forest School of Medicine, Winston-Salem, NC

[27 ]Enanta Pharmaceutical, Watertown, MA

[28 ]Baylor College of Medicine, Houston, TX

Author notes

Corresponding author: Andrew N. Hoofnagle, M.D., Ph.D. Address: Box 357110, University of Washington, Seattle, WA 98115. Phone: (206) 598-6131. Fax: (206) 598-6189

Article

Accession ID: PMC4830481 Pmcid ID: PMC4830481 Pmc-uid ID: 4830481 Manuscript ID: nihpa764456

DOI: 10.1373/clinchem.2015.250563

PMC ID: 4830481

PubMed ID: 26719571

SO-VID: 39614ad3-4eef-40f4-91d6-65d273f8aee8

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