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Generation of human vascularized brain organoids :
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Abstract
<p class="first" id="P1">The aim of this study was to vascularize brain organoids
with a patient’s own endothelial
cells (ECs). Induced pluripotent stem cells (iPSCs) of one UC Davis patient were grown
into whole-brain organoids. Simultaneously, iPSCs from the same patient were differentiated
into ECs. On day 34, the organoid was re-embedded in Matrigel with 250 000 ECs. Vascularized
organoids were grown
<i>in vitro</i> for 3–5 weeks or transplanted into immunodeficient mice on day 54,
and animals were
perfused on day 68. Coating of brain organoids on day 34 with ECs led to robust vascularization
of the organoid after 3–5 weeks
<i>in vitro</i> and 2 weeks
<i>in vivo</i>. Human CD31-positive blood vessels were found inside and in-between
rosettes within
the center of the organoid after transplantation. Vascularization of brain organoids
with a patient’s own iPSC-derived ECs is technically feasible.
</p>