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SARS-CoV-2 is associated with changes in brain structure in UK Biobank.

There is strong evidence for brain-related abnormalities in COVID-19 1-13 . It remains unknown however whether the impact of SARS-CoV-2 infection can be detected in milder cases, and whether this can reveal possible mechanisms contributing to brain pathology. Here, we investigated brain changes in 785 UK Biobank participants (aged 51-81) imaged twice, including 401 cases who tested positive for infection with SARS-CoV-2 between their two scans, with 141 days on average separating their diagnosis and second scan, and 384 controls. The availability of pre-infection imaging data reduces the likelihood of pre-existing risk factors being misinterpreted as disease effects. We identified significant longitudinal effects when comparing the two groups, including: (i) greater reduction in grey matter thickness and tissue-contrast in the orbitofrontal cortex and parahippocampal gyrus, (ii) greater changes in markers of tissue damage in regions functionally-connected to the primary olfactory cortex, and (iii) greater reduction in global brain size. The infected participants also showed on average larger cognitive decline between the two timepoints. Importantly, these imaging and cognitive longitudinal effects were still seen after excluding the 15 cases who had been hospitalised. These mainly limbic brain imaging results may be the in vivo hallmarks of a degenerative spread of the disease via olfactory pathways, of neuroinflammatory events, or of the loss of sensory input due to anosmia. Whether this deleterious impact can be partially reversed, or whether these effects will persist in the long term, remains to be investigated with additional follow up.
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Open Access

Association Between Soft Drink Consumption and Mortality in 10 European Countries

Key Points Question Is regular consumption of soft drinks associated with a greater risk of all-cause and cause-specific mortality? Findings In this population-based cohort study of 451 743 individuals from 10 countries in Europe, greater consumption of total, sugar-sweetened, and artificially sweetened soft drinks was associated with a higher risk of all-cause mortality. Consumption of artificially sweetened soft drinks was positively associated with deaths from circulatory diseases, and sugar-sweetened soft drinks were associated with deaths from digestive diseases. Meaning Results of this study appear to support ongoing public health measures to reduce the consumption of soft drinks.
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Open Access

Once-Weekly Semaglutide in Adults With Alcohol Use Disorder

Key Points Question Does the glucagon-like peptide 1 (GLP-1) receptor agonist semaglutide reduce alcohol consumption and craving in adults with alcohol use disorder (AUD)? Findings In this randomized clinical trial, relative to placebo, low-dose semaglutide reduced the amount of alcohol consumed during a posttreatment laboratory self-administration procedure. Over 9 weeks of treatment, semaglutide led to reductions in some but not all measures of weekly consumption, significantly reduced weekly alcohol craving relative to placebo, and led to greater relative reductions in cigarettes per day in a subgroup of participants with current cigarette use. Meaning These results justify larger clinical trials of incretin therapies for AUD.
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Open Access

Systematic bone tool production at 1.5 million years ago

Recent evidence indicates that the emergence of stone tool technology occurred before the appearance of the genus Homo 1 and may potentially be traced back deep into the primate evolutionary line 2 . Conversely, osseous technologies are apparently exclusive of later hominins from approximately 2 million years ago (Ma) 3, 4 , whereas the earliest systematic production of bone tools is currently restricted to European Acheulean sites 400–250 thousand years ago 5, 6 . Here we document an assemblage of bone tools shaped by knapping found within a single stratigraphic horizon at Olduvai Gorge dated to 1.5 Ma. Large mammal limb bone fragments, mostly from hippopotamus and elephant, were shaped to produce various tools, including massive elongated implements. Before our discovery, bone artefact production in pre-Middle Stone Age African contexts was widely considered as episodic, expedient and unrepresentative of early Homo toolkits. However, our results demonstrate that at the transition between the Oldowan and the early Acheulean, East African hominins developed an original cultural innovation that entailed a transfer and adaptation of knapping skills from stone to bone. By producing technologically and morphologically standardized bone tools, early Acheulean toolmakers unravelled technological repertoires that were previously thought to have appeared routinely more than 1 million years later.

Bone tools shaped by knapping found within Olduvai Gorge in Tanzania precede any other evidence of systematic bone tool production by more than 1 million years.

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A streaming brain-to-voice neuroprosthesis to restore naturalistic communication

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Open Access

Effectiveness of the Influenza Vaccine During the 2024-2025 Respiratory Viral Season

The purpose of this study was to evaluate the effectiveness of the influenza vaccine during the 2024-2025 respiratory viral season. Employees of Cleveland Clinic in employment in Ohio on October 1, 2024, were included. The cumulative incidence of influenza among those in the vaccinated and unvaccinated states was compared over the following 25 weeks. Protection provided by vaccination (analyzed as a time-dependent covariate) was evaluated using Cox proportional hazards regression. Among 53402 employees, 43857 (82.1%) had received the influenza vaccine by the end of the study. Influenza occurred in 1079 (2.02%) during the study. The cumulative incidence of influenza was similar for the vaccinated and unvaccinated states early, but over the course of the study the cumulative incidence of influenza increased more rapidly among the vaccinated than the unvaccinated. In an analysis adjusted for age, sex, clinical nursing job, and employment location, the risk of influenza was significantly higher for the vaccinated compared to the unvaccinated state (HR, 1.27; 95% C.I., 1.07 – 1.51; P = 0.007), yielding a calculated vaccine effectiveness of −26.9% (95% C.I., −55.0 to −6.6%). This study found that influenza vaccination of working-aged adults was associated with a higher risk of influenza during the 2024-2025 respiratory viral season, suggesting that the vaccine has not been effective in preventing influenza this season. Among 53402 working-aged Cleveland Clinic employees, we were unable to find that the influenza vaccine has been effective in preventing infection during the 2024-2025 respiratory viral season.
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Open Access

Acoustic Analysis and Prediction of Type 2 Diabetes Mellitus Using Smartphone-Recorded Voice Segments

Objective To investigate the potential of voice analysis as a prescreening or monitoring tool for type 2 diabetes mellitus (T2DM) by examining the differences in voice recordings between nondiabetic and T2DM individuals. Patients and Methods Total 267 participants diagnosed as nondiabetic (79 women and 113 men) or T2DM (18 women and 57 men) on the basis of American Diabetes Association guidelines were recruited in India between August 30, 2021 and June 30, 2022. Using a smartphone application, participants recorded a fixed phrase up to 6 times daily for 2 weeks, resulting in 18,465 recordings. Fourteen acoustic features were extracted from each recording to analyze differences between nondiabetic and T2DM individuals and create a prediction methodology for T2DM status. Results Significant differences were found between voice recordings of nondiabetic and T2DM men and women, both in the entire dataset and in an age-matched and body mass index (BMI [calculated as the weight in kilograms divided by the height in meters squared])-matched sample. The highest predictive accuracy was achieved by pitch (P<.0001), pitch SD (P<.0001), and relative average pertubation jitter (P=.02) for women, and intensity (P<.0001) and 11-point amplitude perturbation quotient shimmer (apq11, P<0.0001) for men. Incorporating these features with age and BMI, the optimal prediction models achieved accuracies of 0.75±0.22 for women and 0.70±0.10 for men through 5-fold cross-validation in the age-matched and BMI-matched sample. Conclusion Overall, vocal changes occur in individuals with T2DM compared with those without T2DM. Voice analysis shows potential as a prescreening or monitoring tool for T2DM, particularly when combined with other risk factors associated with the condition. Trial Registration clinicaltrials.gov Identifier: CTRI/2021/08/035957
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Open Access

Expression of SARS-CoV-2 spike protein in cerebral Arteries: Implications for hemorrhagic stroke Post-mRNA vaccination

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Open Access

A new Middle Jurassic lagoon margin assemblage of theropod and sauropod dinosaur trackways from the Isle of Skye, Scotland

Although globally scarce, Middle Jurassic dinosaur tracks are known from the Isle of Skye, Scotland, and help indicate the palaeoenvironmental preferences and behaviour of major dinosaur clades. Here, we report an extensive new tracksite from Skye: 131 in-situ dinosaur tracks at Prince Charles’s Point on the Trotternish Peninsula. The tracks occur in multiple horizons of rippled sandstones of the Late Bathonian aged Kilmaluag Formation, part of the Great Estuarine Group, which formed in a locally, shallowly submerged lagoon margin. We assign these tracks to two morphotypes, further divided into four morphotype subgroups, most likely representing large megalosaurid theropods, and sauropods that are either non-neosauropods or basal neosauropods. The trackways, although relatively short, evidence time-averaged milling behaviour, as observed at other tracksites in the Great Estuarine Group. The presence of sequential manus and pes sauropod tracks amends their previous identification by geologists as fish resting burrows, raising the potential that other such structures locally and globally may in fact be dinosaur tracks, and emphasises the predominant occurrence of sauropods in lagoonal palaeoenvironments in the Great Estuarine Group. At Prince Charles’s Point, however, unlike previously described lagoonal assemblages, large theropod trackmakers are more abundant than sauropods.
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Open Access

Neuronal polyunsaturated fatty acids are protective in ALS/FTD

Here we report a conserved transcriptomic signature of reduced fatty acid and lipid metabolism gene expression in a Drosophila model of C9orf72 repeat expansion, the most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia (ALS/FTD), and in human postmortem ALS spinal cord. We performed lipidomics on C9 ALS/FTD Drosophila, induced pluripotent stem (iPS) cell neurons and postmortem FTD brain tissue. This revealed a common and specific reduction in phospholipid species containing polyunsaturated fatty acids (PUFAs). Feeding C9 ALS/FTD flies PUFAs yielded a modest increase in survival. However, increasing PUFA levels specifically in neurons of C9 ALS/FTD flies, by overexpressing fatty acid desaturase enzymes, led to a substantial extension of lifespan. Neuronal overexpression of fatty acid desaturases also suppressed stressor-induced neuronal death in iPS cell neurons of patients with both C9 and TDP-43 ALS/FTD. These data implicate neuronal fatty acid saturation in the pathogenesis of ALS/FTD and suggest that interventions to increase neuronal PUFA levels may be beneficial.
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