Polymorphism of ARHGEF37 gene and its effect on growth traits in F ₁ generation pigs of (Duroc×Saba)♂×[Yorkshire×(Landrace×Saba)]♀

Abstract

[Objective] To reveal the effect of ARHGEF37 gene polymorphisms on the growth traits of pigs, so as to provide a reference for further breeding, development and the utilization of Diansa pig matching line with this gene marker.

[Method] The study used 274 F ₁ generation pigs (Duroc×Saba)♂×[Yorkshire×(Landrace×Saba)]♀ (hereafter DS♂×YLS♀) to screen for polymorphisms in the ARHGEF37 gene by the direct sequencing of PCR amplified products. The effects of genotypes and haplotype combinations of each SNP locus on five growth traits were analyzed by the least square model.

[Result] It was found that 4 SNPs loci were located on exon 8 of ARHGEF37 gene: C1262G, T1293C, G1395A and C1398T The frequency of CC, TT, GA and CC genotype was the highest,followed by allele C, T, G, C and haplotype CTGC. Except for the T1293C locus, the remaining showed a heterozygosity between 0.4552-0.4940, indicating more abundant genetic diversity. The age to 100 kg body weight in the C1262G GG genotype (258.93±2.95 d) was significantly higher than that in the CG genotype (249.87±2.68 d) ( P<0.05, the same below). Measurements of the average daily weight gain in pigs of 30-60 kg showed that the CG genotype (462.44±15.44 g) was significantly higher than that of the CC genotype (409.29±14.30 g) and the TT genotype (445.04±9.70 g) at T1293C was extremely significantly higher than that of the TC genotype (329.33±42.26 g) ( P<0.01). The average daily weight gain in pigs between 30-100 kg in the TT genotype (516.96±6.44 g) was significantly higher than that of the TC and CC genotypes (470.92±28.08 g and 480.64±16.94 g, respectively). In addition, the time to achieve 60 and 100 kg body weight was the shortest in the CTGC/GTGC combination (161.84±5.07 d and 241.00±5.11 d) and the longest in the CTGC/CCGC combination (188.80±11.33 d and 272.60±11.42 d). The average daily gains between 30-60 kg and between 30-100 kg were the highest in the CTGC/GTAT and CTGC/GTGC combinations (479.56±20.66 g and 545.42±19.76 g, respectively), and the lowest in the CTAC/CCGC combination (275.00±84.33 g and 437.00±55.88 g).

[Conclusion] The genetic diversity ofG1395A and C1398T of ARHGEF37 gene is more abundant. The CG genotype of the C1262G locus, TT genotype of the T1293C locus and the combination of CTGC/GTGC haplotype have more influence on the growth traits of the F ₁ generation of DS♂×YLS♀ pigs. The growth traits of individuals carrying the CTGC haplotype are all at a relatively high level.

Abstract

摘要：【目的】揭示 ARHGEF37基因多态性及其对猪生长性状的影响, 为利用该基因标记推进滇撒猪配套系的进 一步选育及开发利用提供参考依据。 【方法】以274头杜撒♂×大长撒♀ F ₁猪为试验材料, 采用PCR扩增产物直接测序 法检测 ARHGEF37基因外显子8多态性, 采用最小二乘模型分析各SNP位点基因型及单倍型组合对5个生长性状的影 响。 【结果】在杜撒♂×大长撒♀ F ₁代猪 ARHGEF37基因外显子8上检测出C1262G、T1293C、G1395A和C1398T 4个SNPs 位点, 分别以CC、TT、GA、CC基因型、等位基因C、T、G、C及单倍型CTGC的频率最高; 除T1293C位点外, 其余各位点 的杂合度在0.4552~0.4940,遗传多样性较丰富。C1262G位点GG基因型个体达100 kg体重日龄(258.93±2.95 d)显著 高于CG基因型个体(249.87±2.68 d) ( P<0.05, 下同), CG基因型个体30~60 kg日增重(462.44±15.44 g)显著高于CC基 因型个体(409.29±14.30 g); T1293C位点TT基因型个体30~60 kg日增重(445.04±9.70 g)极显著高于TC基因型个体 (329.33±42.26 g) ( P<0.01), TT基因型个体30~100 kg日增重(516.96±6.44 g)显著高于TC基因型和CC基因型个体 (470.92±28.08 g和480.64±16.94 g)。此外, 达60 kg体重日龄和达100 kg体重日龄均以CTGC/GTGC组合最短 (161.84±5.07 d和241.00±5.11 d), CTGC/CCGC组合最长(188.80±11.33 d和272.60±11.42 d); 30~60 kg 日增重和 30~100 kg日增重分别以CTGC/GTAT组合和CTGC/GTGC组合最高(479.56±20.66 g和545.42±19.76 g)均以CTAC/CCGC组合最低(275.00±84.33 g和437.00±55.88 g)。 【结论】 ARHGEF37基因的G1395A和C1398T位点的遗传多样性 较丰富;C1262G位点的CG基因型、T1293C位点的TT基因型及CTGC/GTGC单倍型组合对杜撒♂大长撒♀F ₁代生长性 状的影响较大, 携带有CTGC单倍型个体的各生长性状均处于较高水平。