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      Detection of early blight and late blight diseases on tomato leaves using hyperspectral imaging

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      Scientific Reports
      Nature Publishing Group

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          Abstract

          This study investigated the potential of using hyperspectral imaging for detecting different diseases on tomato leaves. One hundred and twenty healthy, one hundred and twenty early blight and seventy late blight diseased leaves were selected to obtain hyperspectral images covering spectral wavelengths from 380 to 1023 nm. An extreme learning machine (ELM) classifier model was established based on full wavelengths. Successive projections algorithm (SPA) was used to identify the most important wavelengths. Based on the five selected wavelengths (442, 508, 573, 696 and 715 nm), an ELM model was re-established. Then, eight texture features (mean, variance, homogeneity, contrast, dissimilarity, entropy, second moment and correlation) based on gray level co-occurrence matrix (GLCM) at the five effective wavelengths were extracted to establish detection models. Among the models which were established based on spectral information, all performed excellently with the overall classification accuracy ranging from 97.1% to 100% in testing sets. Among the eight texture features, dissimilarity, second moment and entropy carried most of the effective information with the classification accuracy of 71.8%, 70.9% and 69.9% in the ELM models. The results demonstrated that hyperspectral imaging has the potential as a non-invasive method to identify early blight and late blight diseases on tomato leaves.

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          Prediction of some quality attributes of lamb meat using near-infrared hyperspectral imaging and multivariate analysis.

          The goal of this study was to explore the potential of near-infrared (NIR) hyperspectral imaging in combination with multivariate analysis for the prediction of some quality attributes of lamb meat. In this study, samples from three different muscles (semitendinosus (ST), semimembranosus (SM), longissimus dorsi (LD)) originated from Texel, Suffolk, Scottish Blackface and Charollais breeds were collected and used for image acquisition and quality measurements. Hyperspectral images were acquired using a pushbroom NIR hyperspectral imaging system in the spectral range of 900-1700 nm. A partial least-squares (PLS) regression, as a multivariate calibration method, was used to correlate the NIR reflectance spectra with quality values of the tested muscles. The models performed well for predicting pH, colour and drip loss with the coefficient of determination (R(2)) of 0.65, 0.91 and 0.77, respectively. Image processing algorithm was also developed to transfer the predictive model in every pixel to generate prediction maps that visualize the spatial distribution of quality parameter in the imaged lamb samples. In addition, textural analysis based on gray level co-occurrence matrix (GLCM) was also conducted to determine the correlation between textural features and drip loss. The results clearly indicated that NIR hyperspectral imaging technique has the potential as a fast and non-invasive method for predicting quality attributes of lamb meat. Copyright © 2011 Elsevier B.V. All rights reserved.
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            Application of Hyperspectral Imaging and Chemometric Calibrations for Variety Discrimination of Maize Seeds

            Hyperspectral imaging in the visible and near infrared (VIS-NIR) region was used to develop a novel method for discriminating different varieties of commodity maize seeds. Firstly, hyperspectral images of 330 samples of six varieties of maize seeds were acquired using a hyperspectral imaging system in the 380–1,030 nm wavelength range. Secondly, principal component analysis (PCA) and kernel principal component analysis (KPCA) were used to explore the internal structure of the spectral data. Thirdly, three optimal wavelengths (523, 579 and 863 nm) were selected by implementing PCA directly on each image. Then four textural variables including contrast, homogeneity, energy and correlation were extracted from gray level co-occurrence matrix (GLCM) of each monochromatic image based on the optimal wavelengths. Finally, several models for maize seeds identification were established by least squares-support vector machine (LS-SVM) and back propagation neural network (BPNN) using four different combinations of principal components (PCs), kernel principal components (KPCs) and textural features as input variables, respectively. The recognition accuracy achieved in the PCA-GLCM-LS-SVM model (98.89%) was the most satisfactory one. We conclude that hyperspectral imaging combined with texture analysis can be implemented for fast classification of different varieties of maize seeds.
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              Predicting quality and sensory attributes of pork using near-infrared hyperspectral imaging.

              Many subjective assessment methods for fresh meat quality are still widely used in the meat industry, making the development of an objective and non-destructive technique for assessing meat quality traits a vital need. In this study, a hyperspectral imaging technique was investigated for objective determination of pork quality attributes. Hyperspectral images in the near infrared region (900-1700 nm) were acquired for pork samples from the longissimus dorsi muscle, and the representative spectral information was extracted from the loin eye area. Several mathematical pre-treatments including first and second derivatives, standard normal variate (SNV) and multiplicative scatter correction (MSC) were applied to examine the influence of spectral variations in predicting pork quality characteristics. Spectral information was used for predicting color features (L, a, b, chroma and hue angle), drip loss, pH and sensory characteristics by partial least-squares regression (PLS-R) models. Independent sets of feature-related wavelengths were selected for predicting each quality attribute. The results showed that color reflectance (L), pH and drip loss of pork meat could be predicted with determination coefficients (R(CV)(2)) of 0.93, 0.87 and 0.83, respectively. The regression coefficients from the PLS-R models at the selected optimal wavelengths were applied in a pixel-wise manner to convert spectral images to prediction maps that display the distribution of attributes within the sample. Results indicated that this technique is a potential tool for rapid assessment of pork quality. Copyright © 2012 Elsevier B.V. All rights reserved.
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                Author and article information

                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group
                2045-2322
                17 November 2015
                2015
                : 5
                : 16564
                Affiliations
                [1 ]College of Biosystems Engineering and Food Science, Zhejiang University , 866 Yuhangtang Road, Hangzhou 310058, China
                Author notes
                Article
                srep16564
                10.1038/srep16564
                4647840
                26572857
                fb26998a-21c0-44bc-9df9-43d85b863958
                Copyright © 2015, Macmillan Publishers Limited

                This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

                History
                : 06 July 2015
                : 15 October 2015
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