RESUMO O presente trabalho teve como objetivo avaliar se a administração contínua de probiótico a frangos de corte provoca alterações a nível enzimático sérico (aspartato aminotransferase, alanina aminotransferase, creatinaquinase, fosfatase alcalina e amilase) que poderiam refletir o estado geral metabólico da ave e, nesse caso, indicando uma interferência benéfica ou adversa do tratamento. Foi verificado também se o probiótico exerce ação sobre a concentração sérica de colesterol total. O probiótico utilizado foi anteriormente preparado no Laboratório de Ornitopatologia, FMVZ, USP, contendo sete amostras bacterianas isoladas de aves livres de patógenos específicos (Laboratório Bio-Vet, Vargem Grande Paulista, SP) e de matrizes adultas sadias. Foram constituídos dois grupos experimentais, com 30 aves cada. O grupo controle recebeu ração comercial e o grupo tratado foi alimentado com ração sem promotores de crescimento, adicionada do probiótico, durante todo o período experimental. As aves receberam o probiótico na dose aproximada de 2,0 x 106 ufc/g de ração. As colheitas de sangue foram realizadas aos 1, 7, 13, 23, 33 e 43 dias de vida. Os resultados demonstraram que a administração de probiótico não interferiu com as atividades das enzimas séricas analisadas e não alterou significativamente a concentração sérica de colesterol.
ABSTRACT The aim of the present investigation was to study the probiotic continuous administration to broilers on serum enzymes activities (aspartate aminotransferase, alanine aminotransferase, creatinekinase, alkaline phosfatase and amylase) that could reflet the general metabolism of the avian, indicating benefities or adversities of the treatment. It was also evaluated the serum concentration of total cholesterol. The probiotic was prepared at Ornitopathology Laboratory, FMVZ, USP. It was elaborated with seven bacterial strains isolated from 50 weeks old specific pathogen free chickens (Bio-Vet Laboratory, Vargem Grande Paulista, SP) and from 35 weeks old breeders. Each experimental group was constituted by 30 broilers. The control group received comercial diet and the treated group was submitted to diets without aditivies, supplemented with aproximately 2.0 x 106 cfu/g diet, during all the experimental period. In the first day, 20 day-old chicks were sacrificed to take the blood samples. The blood samples were taken from six broilers of each group on days 7, 13, 23, 33 and 43. The results demonstrated that the administration of probiotic did not interfere with the analysed serum enzymes activities. It was observed that the treatment did not altered the serum total cholesterol concentration.
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