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      INFLUÊNCIA DA ADMINISTRAÇÃO CONTÍNUA DE PROBIÓTICO A FRANGOS DE CORTE SOBRE ATIVIDADES ENZIMÁTICAS SÉRICAS E CONCENTRAÇÃO DE COLESTEROL SÉRICO* Translated title: INFLUENCE OF PROBIOTIC CONTINUOUS ADMINISTRATION TO BROILERS ON SERUM ENZYMES ACTIVITIES AND SERUM CHOLESTEROL CONCENTRATION

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          Abstract

          RESUMO O presente trabalho teve como objetivo avaliar se a administração contínua de probiótico a frangos de corte provoca alterações a nível enzimático sérico (aspartato aminotransferase, alanina aminotransferase, creatinaquinase, fosfatase alcalina e amilase) que poderiam refletir o estado geral metabólico da ave e, nesse caso, indicando uma interferência benéfica ou adversa do tratamento. Foi verificado também se o probiótico exerce ação sobre a concentração sérica de colesterol total. O probiótico utilizado foi anteriormente preparado no Laboratório de Ornitopatologia, FMVZ, USP, contendo sete amostras bacterianas isoladas de aves livres de patógenos específicos (Laboratório Bio-Vet, Vargem Grande Paulista, SP) e de matrizes adultas sadias. Foram constituídos dois grupos experimentais, com 30 aves cada. O grupo controle recebeu ração comercial e o grupo tratado foi alimentado com ração sem promotores de crescimento, adicionada do probiótico, durante todo o período experimental. As aves receberam o probiótico na dose aproximada de 2,0 x 106 ufc/g de ração. As colheitas de sangue foram realizadas aos 1, 7, 13, 23, 33 e 43 dias de vida. Os resultados demonstraram que a administração de probiótico não interferiu com as atividades das enzimas séricas analisadas e não alterou significativamente a concentração sérica de colesterol.

          Translated abstract

          ABSTRACT The aim of the present investigation was to study the probiotic continuous administration to broilers on serum enzymes activities (aspartate aminotransferase, alanine aminotransferase, creatinekinase, alkaline phosfatase and amylase) that could reflet the general metabolism of the avian, indicating benefities or adversities of the treatment. It was also evaluated the serum concentration of total cholesterol. The probiotic was prepared at Ornitopathology Laboratory, FMVZ, USP. It was elaborated with seven bacterial strains isolated from 50 weeks old specific pathogen free chickens (Bio-Vet Laboratory, Vargem Grande Paulista, SP) and from 35 weeks old breeders. Each experimental group was constituted by 30 broilers. The control group received comercial diet and the treated group was submitted to diets without aditivies, supplemented with aproximately 2.0 x 106 cfu/g diet, during all the experimental period. In the first day, 20 day-old chicks were sacrificed to take the blood samples. The blood samples were taken from six broilers of each group on days 7, 13, 23, 33 and 43. The results demonstrated that the administration of probiotic did not interfere with the analysed serum enzymes activities. It was observed that the treatment did not altered the serum total cholesterol concentration.

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          Most cited references35

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          Probiotics in man and animals.

          There is good evidence that the complex microbial flora present in the gastrointestinal tract of all warm-blooded animals is effective in providing resistance to disease. However, the composition of this protective flora can be altered by dietary and environmental influences, making the host animal susceptible to disease and/or reducing its efficiency of food utilization. What we are doing with the probiotic treatments is re-establishing the natural condition which exists in the wild animal but which has been disrupted by modern trends in conditions used for rearing young animals, including human babies, and in modern approaches to nutrition and disease therapy. These are all areas where the gut flora can be altered for the worse and where, by the administration of probiotics, the natural balance of the gut microflora can be restored and the animal returned to its normal nutrition, growth and health status.
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            Interaction between colonic acetate and propionate in humans.

            Animal studies suggest that propionate, derived from colonic carbohydrate fermentation, may be gluconeogenic and inhibit cholesterol synthesis in the liver. We therefore studied, in six healthy subjects, the effect of rectally infused solutions containing acetate alone (180 mmol), propionate alone (180 mmol), or a mixture of acetate (180 mmol) and propionate (60 mmol). Relative to the control infusion of normal saline, acetate increased serum cholesterol, glucagon, and acetate concentrations and reduced free fatty acids (FFAs) within 30 min. Propionate alone increased serum propionate, glucose, and glucagon with no effects on cholesterol and a delayed fall in FFAs. The addition of propionate to acetate resulted in no significant rise in serum cholesterol. These results are consistent with the hypothesis that colonic propionate is a gluconeogenic substrate in humans and inhibits the utilization of acetate for cholesterol synthesis.
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              Consumption of fermented and nonfermented dairy products: effects on cholesterol concentrations and metabolism.

              The objective of this article was to review existing literature concerning the effects and mechanisms of action of fermented dairy products on serum cholesterol concentrations. Although not without exception, existing evidence from animal and human studies suggests a moderate cholesterol-lowering action of fermented dairy products. Mechanistically, fermented milk has been shown to cause an increase in human gut bacterial content. These bacteria, once resident in the large intestine, are believed to ferment food-derived indigestible carbohydrates. Such fermentation causes increased production of short-chain fatty acids, which decreases circulatory cholesterol concentrations either by inhibiting hepatic cholesterol synthesis or by redistributing cholesterol from plasma to the liver. Furthermore, increased bacterial activity in the large intestine results in enhanced bile acid deconjugation. Deconjugated bile acids are not well absorbed by the gut mucosa and are excreted. Consequently, cholesterol, being a precursor of bile acids, is utilized to a greater extent for de novo bile acid synthesis. These actions combined are proposed as contributing mechanisms to the association of fermented milk consumption with decreased circulating cholesterol concentrations.
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                Author and article information

                Journal
                aib
                Arquivos do Instituto Biológico
                Arq. Inst. Biol.
                Instituto Biológico (São Paulo, SP, Brazil )
                0020-3653
                1808-1657
                December 2001
                : 68
                : 2
                : 11-17
                Affiliations
                [1] Descalvado SP orgnameLaboratório de Patologia Avícola de Descalvado orgdiv1Instituto Biológico Brasil
                [2] São Paulo SP orgnameFMVZ, USP orgdiv1Departamento de Patologia Veterinária
                Article
                S1808-16572001000200011 S1808-1657(01)06800200011
                10.1590/1808-1657v68n2p0112001
                f81bb514-13e1-4332-b4c9-ab9a72b678c2

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 18 July 2000
                : 11 January 2001
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 35, Pages: 7
                Product

                SciELO Brazil


                serum enzymes,Probiotic,colesterol.,enzimas séricas,frango de corte,Probiótico,cholesterol.,broiler

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