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Abstract
<p xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="first" dir="auto"
id="d20720972e93">Extramammary Paget's disease (EMPD) is a rare skin cancer that mainly
occurs in apocrine
sweat gland-rich areas in elderly people. The prognosis of metastatic EMPD is unfavorable
because of the lack of fully effective systemic therapies. However, the difficulty
in establishing a model of EMPD has hampered basic research for exploring its pathogenesis
and optimal treatments. Here, we established for the first time an EMPD cell line
(named KS-EMPD-1) from a primary tumor on the left inguinal region of an 86-year-old
Japanese male. The cells were successfully maintained for more than 1 year, with a
doubling time of 31.2 ± 0.471 h. KS-EMPD-1 exhibited constant growth, spheroid formation,
and invasiveness, and was confirmed to be identical to the original tumor by short
tandem repeat analyses, whole exome sequencing, and immunohistochemistry (CK7+CK20-GCDFP15+).
Western blotting of the cells revealed the protein expression of HER2, NECTIN4, and
TROP2, which have recently attracted attention as potential therapeutic targets for
EMPD. KS-EMPD-1 was highly sensitive to docetaxel and paclitaxel on chemosensitivity
test. The KS-EMPD-1 cell line is a promising resource for basic and preclinical research
on EMPD to better define the tumor characteristics and treatment strategy of this
rare cancer.
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