There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

1. Using the whole cell configuration of the patch clamp technique, we studied the potential blocking effects of gadolinium (1 microM to 1 mM) on potassium currents: I(KR), I(KS) and I(K1). The study was performed on guinea-pig isolated ventricular myocytes. 2. The background current, I(K1) was insensitive to Gd3+. Thus, we found that no obvious screening of surface charges was visible with concentrations of Gd3+ up to 100 microM. 3. By use of three different protocols: tail currents fitting, analysis of envelope of tails and electrophysiological dissection, we found that I(KR) was the only component of IK that was sensitive to Gd3+. The sensitivity was apparently different depending on the protocol used. 4. Comparison of the results obtained with the different protocols revealed that the rapid component of I(KR) is more sensitive to Gd3+ than the slow one. 5. Of the different protocols used to distinguish between I(KR) and I(KS), the electrophysiological dissection seems to be the more accurate.

Related collections

Author and article information

Journal

PubMed ID:: 9641553

PMC ID:: 1565389

DOI:: 10.1038/sj.bjp.0701835

ScienceOpen disciplines: Chemistry

Keywords: Animals,Dose-Response Relationship, Drug,Gadolinium,pharmacology,Guinea Pigs,Heart Ventricles,cytology,drug effects,In Vitro Techniques,Ion Transport,Myocardium,Patch-Clamp Techniques,Potassium Channels,Rats,Tail

Data availability:

ScienceOpen disciplines: Chemistry

Keywords: Animals, Dose-Response Relationship, Drug, Gadolinium, pharmacology, Guinea Pigs, Heart Ventricles, cytology, drug effects, In Vitro Techniques, Ion Transport, Myocardium, Patch-Clamp Techniques, Potassium Channels, Rats, Tail

Comments

Comment on this article

scite_

0

Smart Citations

0

Citing PublicationsSupportingMentioningContrasting

View Citations

See how this article has been cited at scite.ai

scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.