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      Microfluidic Systems for Pathogen Sensing: A Review

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          Abstract

          Rapid pathogen sensing remains a pressing issue today since conventional identification methodsare tedious, cost intensive and time consuming, typically requiring from 48 to 72 h. In turn, chip based technologies, such as microarrays and microfluidic biochips, offer real alternatives capable of filling this technological gap. In particular microfluidic biochips make the development of fast, sensitive and portable diagnostic tools possible, thus promising rapid and accurate detection of a variety of pathogens. This paper will provide a broad overview of the novel achievements in the field of pathogen sensing by focusing on methods and devices that compliment microfluidics.

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          Most cited references141

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          Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.

          L Gold, C Tuerk (1990)
          High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds exponentially enrich the population for the highest affinity species that can be clonally isolated and characterized. In particular one eight-base region of an RNA that interacts with the T4 DNA polymerase was chosen and randomized. Two different sequences were selected by this procedure from the calculated pool of 65,536 species. One is the wild-type sequence found in the bacteriophage mRNA; one is varied from wild type at four positions. The binding constants of these two RNA's to T4 DNA polymerase are equivalent. These protocols with minimal modification can yield high-affinity ligands for any protein that binds nucleic acids as part of its function; high-affinity ligands could conceivably be developed for any target molecule.
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            Pathogen detection: a perspective of traditional methods and biosensors.

            Olivier Lazcka, F. del Campo, F. Xavier Munoz (2007)
            The detection of pathogenic bacteria is key to the prevention and identification of problems related to health and safety. Legislation is particularly tough in areas such as the food industry, where failure to detect an infection may have terrible consequences. In spite of the real need for obtaining analytical results in the shortest time possible, traditional and standard bacterial detection methods may take up to 7 or 8 days to yield an answer. This is clearly insufficient, and many researchers have recently geared their efforts towards the development of rapid methods. The advent of new technologies, namely biosensors, has brought in new and promising approaches. However, much research and development work is still needed before biosensors become a real and trustworthy alternative. This review not only offers an overview of trends in the area of pathogen detection but it also describes main techniques, traditional methods, and recent developments in the field of pathogen bacteria biosensors.
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              Micro total analysis systems. 2. Analytical standard operations and applications.

              Pierre-Alain Auroux, Dimitri Iossifidis, Darwin R. Reyes … (2002)
              Article 0 comments Cited 171 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Sensors (Basel)
                Title: Sensors (Basel, Switzerland)
                Publisher: Molecular Diversity Preservation International (MDPI)
                ISSN (Electronic): 1424-8220
                Publication date Collection: 2009
                Publication date (Electronic): 17 June 2009
                Volume: 9
                Issue: 6
                Pages: 4804-4823
                Affiliations
                [1 ] Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
                [2 ] Division of Nano-System-Technologies, Austrian Research Centers GmbH – ARC, Donau-City-Street 1, 1220 Vienna, Austria
                Author notes
                [* ]Author to whom correspondence should be addressed; E-Mail: peter.ertl@ 123456arcs.ac.at ; Tel.: +43-(0)50550-4305; Fax: +43-(0)50550-4399
                Article
                Publisher ID: sensors-09-04804
                DOI: 10.3390/s90604804
                PMC ID: 3291940
                PubMed ID: 22408555
                SO-VID: f15a5e4e-399a-4cad-a4f7-cd8ff20890a9
                Copyright © © 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
                License:

                This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).

                History
                Date received : 28 April 2009
                Date revision received : 4 June 2009
                Date accepted : 8 June 2009
                Categories
                Subject: Review

                ScienceOpen disciplines: Biomedical engineering
                Keywords: lab-on-a-chip,pathogen sensing,microfluidics
                Data availability:
                ScienceOpen disciplines: Biomedical engineering
                Keywords: lab-on-a-chip, pathogen sensing, microfluidics

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