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      Aerobic stability in corn silage (Zea mays L.) ensiled with different microbial additives Translated title: Estabilidade aerobica em silagem de milho (Zea mays L.) ensilada com diferentes aditivos microbianos

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          Abstract

          ABSTRACT. This study aimed to assess different microbial additives, regarding the efficiency of aerobic stability in corn silages. The corn hybrid used for silages production was the DKB 310. The treatments consisted of: 1) control treatment without any microbial additive; 2) Treatment with LPPA composed of: Lactobacillus plantarum CCT 0580 3.1 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 3.1 x 1010 CFU g-1; 3) Treatment with Inoculum, composed of Bacillus subtilis CCT 0089 3.0 x 109 CFU g-1, Lactobacillus plantarum CCT 0580 1.2 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 1.5 x 1010 CFU g-1 and 4) Treatment LB, composed only of Lactobacillus buchneri CCT 3746 2.6 x 1010 CFU g-1. The experimental design was completely randomized with five replicates for each treatment. For the parameters evaluated daily, we used the split plot design, in which the different silages were assigned to the plots and the time of exposure to air was assigned to the subplots. There was no difference of additives on silage pH in any of the evaluation days. The control silage reached higher temperatures indicating greater vulnerability. All additives had no aerobic instability. Silages made with the use of microbial additives were effective in maintaining the aerobic stability.

          Translated abstract

          RESUMO. O objetivo deste trabalho foi avaliar diferentes aditivos microbianos, no tocante a eficiência da estabilidade aeróbia em silagens de milho. O híbrido de milho utilizado para cultivo e posterior confecção das silagens foi o DKB 310. Os tratamentos impostos às silagens foram: 1) Tratamento Controle, sem nenhum aditivo microbiano. 2) Tratamento com LPPA composto por: Lactobacillus plantarum CCT 0580 3,1x1010 UFC/g e Propionibacterium acidipropionici CCT 4843 3,1 x 1010 UFC g-1; 3) Tratamento com Inoculum, composto por: Bacillus subtilis CCT 0089 3,0 x 109 UFC g-1, Lactobacillus plantarum CCT 0580 1,2 x 1010 UFC g-1 e Propionibacterium acidipropionici CCT 4843 1,5 x 1010 UFC g-1 e 4) Tratamento LB, composto apenas por Lactobacillus buchneri CCT 3746 2,6 x 1010 UFC g-1. O delineamento experimental foi inteiramente casualizado, com cinco repetições para cada tratamento. Para os parâmetros avaliados diariamente, utilizou-se o esquema de parcelas subdivididas, em que os fatores das parcelas foram as silagens e o fator atribuído à subparcela, o tempo de exposição ao ar. Não houve diferença dos aditivos sobre o pH da silagem em nenhum dos dias de avaliação. A silagem controle atingiu valores de temperatura maiores indicando maior vulnerabilidade. Todos os aditivos não apresentaram instabilidade aeróbia. As silagens confeccionadas com uso dos aditivos microbianos foram eficientes em manter a estabilidade aeróbia.

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          Most cited references19

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          Silage microbiology and its control through additives

          Ensiling is a method of preserving a moist crop. A moist crop can support the growth of a wide range of microorganisms, most of which will degrade the nutrient value to livestock. However, ensiling generally controls microbial activity by a combination of an anaerobic environment and a natural fermentation of sugars by lactic acid bacteria on the crop. This fermentation and the resulting low pH primarily suppress the growth of other anaerobic microorganisms. The fermentation can also inhibit yeasts, molds and aerobic bacteria, but the anaerobic environment is essential to preventing most of the spoilage microorganisms from growing. Inoculants have become the dominant additives for making silage. Homofermentative strains help guarantee a rapid suppression of anaerobic stains early in storage, increase dry matter recovery and have improved animal performance by means that we do not fully understand. Inoculants containing Lactobacillus buchneri, a heterofermentative species capable of fermenting lactic acid to acetic, are recent additives. The added acetic acid inhibits yeast and mold growth, increasing aerobic stability of silages at feeding.
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            The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage.

            Several microorganisms and one chemical preservative were tested for their effects on the fermentation and aerobic stability of corn silage. Whole-plant corn (one-half milk line, 31.3% dry matter) was ensiled in quadruplicate 20-L laboratory silos untreated or after the following treatments: Lactobacillus buchneri at 1 x 10(5) and 1 x 10(6) cfu/g of fresh forage; two different strains of L. plantarum, each at 1 x 10(6) cfu/g; and a buffered propionic acid-based product at 0.1% of fresh forage weight. After 100 d of ensiling, silage treated with L. buchneri (1 x 10(6) cfu/g) had a lower concentration of lactic acid compared with the untreated silage, but was similar to other treated silages. The silage treated with the high (1 x 10(6) cfu/g), but not the moderate rate (1 x 10(5) cfu/g) of L. buchneri also had a greater concentration of acetic acid (3.60%) and less yeasts (2.01 log cfu/g) when compared with other treatments (average of 1.88% acetic acid and 5.85 log cfu of yeasts/g). Silages treated with L. plantarums, the moderate rate of L. buchneri, and the chemical preservative took longer to heat than untreated silage when exposed to air, but improvements were numerically small (6.3 to 10.5 h). In contrast, silage treated with the high rate of L. buchneri never heated throughout a 900-h period of monitoring. Inoculating corn silage with 1 x 10(6) cfu/g of L. buchneri resulted in a more heterolactic fermentation and dramatically improved the aerobic stability of corn silage.
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              A simple method for the analysis of particle sizes of forage and total mixed rations.

              A simple separator was developed to determine the particle sizes of forage and TMR that allows for easy separation of wet forage into three fractions and also allows plotting of the particle size distribution. The device was designed to mimic the laboratory-scale separator for forage particle sizes that was specified by Standard S424 of the American Society of Agricultural Engineers. A comparison of results using the standard device and the newly developed separator indicated no difference in ability to predict fractions of particles with maximum length of less than 8 and 19 mm. The separator requires a small quantity of sample (1.4 L) and is manually operated. The materials on the screens and bottom pan were weighed to obtain the cumulative percentage of sample that was undersize for the two fractions. The results were then plotted using the Weibull distribution, which proved to be the best fit for the data. Convenience samples of haycrop silage, corn silage, and TMR from farms in the northeastern US were analyzed using the forage and TMR separator, and the range of observed values are given.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                asas
                Acta Scientiarum. Animal Sciences
                Acta Sci., Anim. Sci.
                Editora da Universidade Estadual de Maringá - EDUEM (Maringá, PR, Brazil )
                1806-2636
                1807-8672
                December 2017
                : 39
                : 4
                : 357-362
                Affiliations
                [1] Londrina Paraná orgnameUniversidade Estadual de Londrina orgdiv1Departamento de Zootecnia Brazil
                [5] Jaboticabal orgnameUniversidade Estadual Paulista orgdiv1Programa de Pós-graduação em Zootecnia Brazil
                [4] Campo Grande São Paulo orgnameFaculdade de Medicina Veterinária e Zootecnia orgdiv1Programa de Pós-graduação em Ciência Animal Brazil
                [3] Vacaria Rio Grande do Sul orgnameInstituto Federal do Rio Grande do Sul Brazil
                [2] Alto Paraná Paraná orgnameInstituto Paranaense de Assistência Técnica e Extensão Rural Brazil
                Article
                S1807-86722017000400357
                10.4025/actascianimsci.v39i4.34426
                ece3dcf5-271b-42c1-beac-2f8f39c19140

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 10 May 2017
                : 30 November 2016
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 20, Pages: 6
                Product

                SciELO Brazil


                ensilagem,qualidade nutricional,aerobiosis,aerobiose,fermentação,silage,fermentation,nutritional quality

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