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      Alterations in muscular enzymes of horses competing long-distance endurance rides under tropical climate Translated title: Alterações em enzimas musculares de cavalos em competições de enduro de longa distância em clima tropical

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          Abstract

          This study tried to monitor the alterations of muscle enzymes activity - creatinokinase (CK), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST) - in a group of horses that participated of 70 and 100km distance rides in five competitions of an annual endurance championship under tropical climate. Pre ride levels (U/l) were 245.13±9.84 for CK, 496.61±14.76 for LDH, and 328.95±8.65 for AST. When compared to these levels, the results revealed a significant decrease in all enzymes activities in the first moment of the rides. Peak levels, significantly different, were reached, immediately after rides by CK (413.591±50.75); 24 hours post rides by LDH (628.61±33.30); and 48 hours after rides by AST (389.89±16.96). Monitoring of recovery period revealed different behavior among enzymes activities with CK values returning to pre ride values (279.61±23.05) 24 hours post rides, while LDH and AST values returned to pre ride values (505.25±33.78 and 359.35±24.90, respectively) 72 hours post rides. These data revealed different alterations in concentration of muscular enzymes in endurance horses directly related to the duration of the effort.

          Translated abstract

          Estudaram-se as alterações de atividade das enzimas musculares creatino quinase (CK), lactato desidrogenase (LDH) e aspartato aminotransferase (AST) em um grupo de cavalos que utilizados em provas de enduro de 70 e 100km de distância, em cinco competições. Os valores (U/l) basais (antes da largada) foram 245,13±9,84 para CK, 496,61±14,76 para LDH e 328,95±8,65 para AST. Todas as atividades das enzimas decresceram no primeiro momento das provas (~30km). Valores de pico, significativamente diferentes, foram alcançados para CK (413,59±50,75) imediatamente após 70km de distância; 24 horas após para LDH (628,61±33,30); e 48 horas após as provas para AST (389,89±16,96). A monitoração do período de recuperação revelou diferente comportamento entre as concentrações enzimáticas com CK retornando aos valores basais 24 horas pós-provas (279,61 ± 23,05). LDH e AST retornaram aos valores basais, 72 horas pós-provas (505,25±33,78 e 359,35±24,90, respectivamente). Os dados obtidos revelaram diferentes alterações na concentração de enzimas musculares de cavalos de enduro, diretamente relacionadas com a duração do esforço.

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          Most cited references19

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          Muscle damage and endurance events.

          Intensive training for and competition in endurance events like the marathon are accompanied by injury to fibres in the active skeletal muscles. Evidence for the injury comes from the increases in intramuscular enzymes and myoglobin found in the blood following the exercise, from the subjective sensation of soreness in the muscles in the post-exercise period, and from direct histological examination of samples of the damaged muscles. Histological studies demonstrate that some muscle fibres undergo degenerative changes following the exercise; the necrosis is accomplished by macrophages and other phagocytic cells that invade the injured cells and the adjacent interstitium. Following the degeneration the fibers appear to be regenerated so that there is not a net loss of fibres. Precisely what initiates the cellular damage is not known, but hypotheses suggested include, 'metabolic overload' and 'mechanical strain'. Eccentric contractions are known to cause the greater amount of damage in muscles, which suggests that high local tensions in fibres may be more important than metabolic considerations in the aetiology of the injury. Training reduces the magnitude of the damage that occurs in response to a given exercise task, although competitors in endurance events may demonstrate chronic muscle injury because of increasing training intensities. Other than training, there is no compelling evidence that any drug treatment or preventative measures will lessen this form of injury.
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            The mechanisms relating to increases in plasma enzymes and isoenzymes in diseases of animals.

            J W Boyd (1983)
            Changes in plasma or serum enzymes and isoenzymes are useful indicators of tissue damage in many diseases. Enzyme increases are usually related to leakage of enzymes from damaged cells. The amount of increase depends on factors such as the concentration of enzyme in the cells, the rate of leakage of enzyme from cells and the rate of clearance of enzyme from plasma. There are important species variations in the tissue concentrations and plasma clearance rates of enzymes and isoenzymes.
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              Determinação das atividades séricas de creatina quinase, lactato desidrogenase e aspartato aminotransferase em eqüinos de diferentes categorias de atividade

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                abmvz
                Arquivo Brasileiro de Medicina Veterinária e Zootecnia
                Arq. Bras. Med. Vet. Zootec.
                Universidade Federal de Minas Gerais, Escola de Veterinária (Belo Horizonte )
                1678-4162
                June 2008
                : 60
                : 3
                : 543-549
                Affiliations
                [1 ] UNESP
                [2 ] Universidade Federal de Goiás Brazil
                [3 ] Santé Laboratório
                [4 ] UnB
                [5 ] UNESP
                Article
                S0102-09352008000300004
                10.1590/S0102-09352008000300004
                e5c9b20c-1cfb-4aa9-96a3-81c254d10402

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0102-0935&lng=en
                Categories
                VETERINARY SCIENCES

                General veterinary medicine
                horse,endurance,enzymes,muscle,cavalo,enduro,enzimas,músculo
                General veterinary medicine
                horse, endurance, enzymes, muscle, cavalo, enduro, enzimas, músculo

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