For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
4
views
33
references
 Top references
cited by
18
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      156
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Enhancing PD-1 Gene Silence in T Lymphocytes by Comparing the Delivery Performance of Two Inorganic Nanoparticle Platforms

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Suitable carriers are crucial to RNAi applications for cancer genotherapy and T-cell immunotherapy. In this research, we selected two extensively-investigated biocompatible inorganic nanoparticle carriers, i.e., layered double hydroxide (LDH) and lipid-coated calcium phosphate (LCP) and then compared their efficacy for siRNA delivery in T cells, in order to understand which carrier is more efficient in delivering functional programmed cell death protein 1 siRNA (PD-1 siRNA) to suspended T lymphocytes. Both LDH and LCP nanoparticles quickly delivered gene segment to mouse T cell lines (EL4), while the LCP nanoparticles exhibited more cellular uptake and higher PD-1 gene silence efficiency. We further demonstrated that LCP nanoparticles successfully reduced the expression of PD-1 in human ex vivo tumor infiltrating lymphocytes (TILs). Thus, LCP nanoparticles can be used as a better nano-carrier for gene therapy in lymphocytes, especially in regards to TIL-related cancer immunotherapy.

          Related collections

          Most cited references33

          • Record: found
          • Abstract: found
          • Article: not found

          The possible "proton sponge " effect of polyethylenimine (PEI) does not include change in lysosomal pH.

          Maria A Mattebjerg, Rikke Benjaminsen, Jonas Henriksen … (2012)
          Polycations such as polyethylenimine (PEI) are used in many novel nonviral vector designs and there are continuous efforts to increase our mechanistic understanding of their interactions with cells. Even so, the mechanism of polyplex escape from the endosomal/lysosomal pathway after internalization is still elusive. The "proton sponge " hypothesis remains the most generally accepted mechanism, although it is heavily debated. This hypothesis is associated with the large buffering capacity of PEI and other polycations, which has been interpreted to cause an increase in lysosomal pH even though no conclusive proof has been provided. In the present study, we have used a nanoparticle pH sensor that was developed for pH measurements in the endosomal/lysosomal pathway. We have carried out quantitative measurements of lysosomal pH as a function of PEI content and correlate the results to the "proton sponge " hypothesis. Our measurements show that PEI does not induce change in lysosomal pH as previously suggested and quantification of PEI concentrations in lysosomes makes it uncertain that the "proton sponge " effect is the dominant mechanism of polyplex escape.
          Article 0 comments Cited 177 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Inorganic nanoparticles as carriers for efficient cellular delivery

            Zhi Ping Xu, Qing Hua Zeng, Gao Qing Lu … (2006)
            Article 0 comments Cited 169 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Acid-Activatable Versatile Micelleplexes for PD-L1 Blockade-Enhanced Cancer Photodynamic Immunotherapy.

              Qi Yin, Zhaocai Zhou, Yaping Li … (2016)
              Photodynamic therapy (PDT) has emerged as a promising clinical modality for cancer therapy due to its ability to initiate an antitumor immune response. However, PDT-mediated cancer immunotherapy is severely impaired by tumor-cell immunosuppression of host T cell antitumor activity through the programmed cell death 1 ligand (PD-L1) and programmed cell death receptor 1 (PD-1) (PD-L1-PD-1) immune checkpoint pathway. Here, we demonstrate that PDT-mediated cancer immunotherapy can be augmented by PD-L1 knockdown (KD) in tumor cells. We rationally designed a versatile micelleplex by integrating an acid-activatable cationic micelle, photosensitizer (PS), and small interfering RNA (siRNA). The micelleplex was inert at physiological pH conditions and activated only upon internalization in the acidic endocytic vesicles of tumor cells for fluorescence imaging and PDT. Compared to PDT alone, the combination of PDT and PD-L1 KD showed significantly enhanced efficacy for inhibiting tumor growth and distant metastasis in a B16-F10 melanoma xenograft tumor model. These results suggest that acid-activatable micelleplexes utilizing PDT-induced cancer immunotherapy are more effective when combined with siRNA-mediated PD-L1 blockade. This study could provide a general strategy for enhancing the therapy efficacy of photodynamic cancer therapy.
              Article 0 comments Cited 149 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Nanomaterials (Basel)
                Journal ID (iso-abbrev): Nanomaterials (Basel)
                Journal ID (publisher-id): nanomaterials
                Title: Nanomaterials
                Publisher: MDPI
                ISSN (Electronic): 2079-4991
                Publication date (Electronic): 28 January 2019
                Publication date Collection: February 2019
                Volume: 9
                Issue: 2
                Electronic Location Identifier: 159
                Affiliations
                [1 ]Australian Institute for Bioengineering and Nanotechnology, the University of Queensland, St. Lucia 4072, QLD, Australia; yanheng.wu@ 123456uq.net.au (Y.W.); l.li2@ 123456uq.edu.au (L.L.)
                [2 ]School of Biomedical Sciences, the University of Queensland, St. Lucia 4072, QLD, Australia; chen.chen@ 123456uq.edu.au
                Author notes
                [* ]Correspondence: w.gu@ 123456uq.edu.au (W.G.); gordonxu@ 123456uq.edu.au (Z.P.X.); Tel.: +61-7-334-63809 (Z.P.X.)
                Author information
                https://orcid.org/0000-0003-3360-1390
                https://orcid.org/0000-0003-2104-534X
                https://orcid.org/0000-0001-6070-5035
                Article
                Publisher ID: nanomaterials-09-00159
                DOI: 10.3390/nano9020159
                PMC ID: 6410115
                PubMed ID: 30696033
                SO-VID: e51b0460-7bc4-48e0-86fc-98aefa6ed94d
                Copyright © © 2019 by the authors.
                License:

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 18 December 2018
                Date accepted : 24 January 2019
                Categories
                Subject: Article

                Keywords: layered double hydroxide (ldh) nanoparticle,lipid-coated calcium phosphate (lcp) nanoparticle,programmed cell death protien-1 (pd-1),human tumor infiltrating lymphocytes (tils),pd-1 gene silencing,el4 cells
                Data availability:
                Keywords: layered double hydroxide (ldh) nanoparticle, lipid-coated calcium phosphate (lcp) nanoparticle, programmed cell death protien-1 (pd-1), human tumor infiltrating lymphocytes (tils), pd-1 gene silencing, el4 cells

                Comments

                Comment on this article

                scite_

                Similar content156

                See all similar

                Cited by18

                See all cited by

                Most referenced authors395

                See all reference authors