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Abstract
DNA clones of the wild-type p53 proto-oncogene inhibit the ability of E1A plus ras
or mutant p53 plus ras-activated oncogenes to transform primary rat embryo fibroblasts.
The rare clones of transformed foci that result from E1A plus ras plus wild-type p53
triple transfections all contain the p53 DNA in their genome, but the great majority
fail to express the p53 protein. The three cell lines derived from such foci that
express p53 all produce mutant p53 proteins with properties similar or identical to
transformation-activated p53 proteins. The p53 mutants selected in this fashion (transformation
in vitro) resemble the p53 mutants selected in tumors (in vivo). These results suggest
that the p53 proto-oncogene can act negatively to block transformation.