For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
3
views
51
references
 Top references
cited by
0
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
1 collections
    0
    shares
      706
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Expression analysis of m6A-related genes in various tissues of Meishan pigs at different developmental stages

      rapid-communication

      Read this article at

      SciELO
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          ABSTRACT To characterize the N6-methyladenosine (m6A)-related gene expression profiles in various tissues of Meishan pigs at different stages, m6A modification-related genes (METTL3, METTL14, METTL16, WTAP, RBM15, and FTO) were detected from newborn to physical maturity of Meishan pigs at eight important developmental stages (1, 7, 14, 21, 28, 35, 134, and 158 days old). The expression of m6A-related genes was tissue-specific. Furthermore, the level of METTL3 messenger RNA (mRNA) was higher on day 35 than in other stages in most tissues, and the expression of METTL14 increased after day 35, and FTO exhibited a peak on day 14 in muscle, intestine, lymph nodes, thymus, and kidney. This study provided a reference for an in-depth study of the expression patterns of m6A modification-related genes in Meishan pigs.

          Related collections

          Most cited references51

          • Record: found
          • Abstract: found
          • Article: not found

          N6-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO

          Guifang Jia, Ye Fu, Xu Zhao … (2011)
          We report here that FTO (fat mass and obesity-associated protein) exhibits efficient oxidative demethylation activity of abundant N 6-methyladenosine (m6A) residues in RNA in vitro. FTO knockdown with siRNA led to an increased level of m6A in mRNA, whereas overexpression of FTO resulted in a decreased level of m6A in human cells. We further show that FTO partially colocalizes with nuclear speckles, supporting m6A in nuclear RNA as a physiological substrate of FTO.
          Article 0 comments Cited 1128 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility.

            Guanqun Zheng, John Dahl, Yamei Niu … (2013)
            N(6)-methyladenosine (m(6)A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as another mammalian demethylase that oxidatively reverses m(6)A in mRNA in vitro and in vivo. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice have increased m(6)A in mRNA and are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1,551 differentially expressed genes that cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. The discovery of this RNA demethylase strongly suggests that the reversible m(6)A modification has fundamental and broad functions in mammalian cells. Copyright © 2013 Elsevier Inc. All rights reserved.
            Article 0 comments Cited 1061 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase

              Xiao-Li Ping, Bao-Fa Sun, Lu Wang … (2014)
              The methyltransferase like 3 (METTL3)-containing methyltransferase complex catalyzes the N6-methyladenosine (m6A) formation, a novel epitranscriptomic marker; however, the nature of this complex remains largely unknown. Here we report two new components of the human m6A methyltransferase complex, Wilms' tumor 1-associating protein (WTAP) and methyltransferase like 14 (METTL14). WTAP interacts with METTL3 and METTL14, and is required for their localization into nuclear speckles enriched with pre-mRNA processing factors and for catalytic activity of the m6A methyltransferase in vivo. The majority of RNAs bound by WTAP and METTL3 in vivo represent mRNAs containing the consensus m6A motif. In the absence of WTAP, the RNA-binding capability of METTL3 is strongly reduced, suggesting that WTAP may function to regulate recruitment of the m6A methyltransferase complex to mRNA targets. Furthermore, transcriptomic analyses in combination with photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) illustrate that WTAP and METTL3 regulate expression and alternative splicing of genes involved in transcription and RNA processing. Morpholino-mediated knockdown targeting WTAP and/or METTL3 in zebrafish embryos caused tissue differentiation defects and increased apoptosis. These findings provide strong evidence that WTAP may function as a regulatory subunit in the m6A methyltransferase complex and play a critical role in epitranscriptomic regulation of RNA metabolism.
              Article 0 comments Cited 782 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (publisher-id): rbz
                Title: Revista Brasileira de Zootecnia
                Abbreviated Title: R. Bras. Zootec.
                Publisher: Sociedade Brasileira de Zootecnia (Viçosa, MG, Brazil )
                ISSN (Print): 1516-3598
                ISSN (Electronic): 1806-9290
                Publication date (Print and electronic): 2023
                Volume: 52
                Electronic Location Identifier: e20210149
                Affiliations
                [2] Yangzhou orgnameYangzhou University orgdiv1Joint International Research Laboratory of Agriculture & Agri-Product Safety China
                [1] Yangzhou orgnameYangzhou University orgdiv1College of Animal Science and Technology orgdiv2Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design China
                Article
                Publisher ID: S1516-35982023000100401 Publisher ID: S1516-3598(23)05200000401
                DOI: 10.37496/rbz5220210149
                SO-VID: e2d2610e-3a8b-4b36-bf40-a0f1056abf50
                License:

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                Date received : 20 August 2021
                Date accepted : 12 September 2022
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 51, Pages: 0
                Product

                SciELO Brazil


                Keywords: weaned piglets,FTO,METTL3,tissue expression profile
                Data availability:
                Keywords: weaned piglets, FTO, METTL3, tissue expression profile

                Comments

                Comment on this article