Immobilization of IMP-1 metallo-beta-lactamase on Fe3O4@SiO2 as nanobiocatalyst for degradation of beta-lactam antibiotics in wastewater

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Abstract

Residues of antibiotics in water resources and wastewater have been significant environmental and public health problems. The current study developed a high-efficiency enzymatic nanobiocatalyst for the degradation of beta-lactam antibiotics. For this purpose, metallo-beta-lactamase IMP-1 was obtained by the cloned blaIMP gene overexpressed in Escherichia coli. 2.6 mg purified enzyme was used for immobilization on 100 mg modified Fe3O4 @ SiO2 magnetic nanoparticles. Immobilized IMP-1 showed similar storage stability to the free enzyme. The optimum temperatures and enzyme activity pH for free and immobilized enzymes were 70 °C and 60 °C, 7.5 and 6.5, respectively. In addition, after 15 reaction cycles, 80 percent of the enzyme activity was retained, according to a reusability analysis of the immobilized enzyme. For free and immobilized enzymes, the highest catalytic activity was observed for penicillin G and cephalexin, whereas Vmax/Km value for ceftriaxone was 3-fold (free enzyme) to 10-fold (immobilized enzyme) lower than for penicillin G. Also, the results showed that the immobilized IMP-1 on magnetic nanoparticles has an excellent ability to remove beta-lactam antibiotics from aqueous solutions. Thus, i could be an appropriate choice for removing beta-lactam antibiotics from pharmaceutical industry wastewater.

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Most cited references 69

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Marion Bradford (1976)

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Enzyme immobilisation in biocatalysis: why, what and how.

Phillip R. Sheldon, Sander van den Heuvel (2013)

In this tutorial review, an overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented. The importance of biocatalysis in the context of green and sustainable chemicals manufacture is discussed and the necessity for immobilisation of enzymes as a key enabling technology for practical and commercial viability is emphasised. The underlying reasons for immobilisation are the need to improve the stability and recyclability of the biocatalyst compared to the free enzyme. The lower risk of product contamination with enzyme residues and low or no allergenicity are further advantages of immobilised enzymes. Methods for immobilisation are divided into three categories: adsorption on a carrier (support), encapsulation in a carrier, and cross-linking (carrier-free). General considerations regarding immobilisation, regardless of the method used, are immobilisation yield, immobilisation efficiency, activity recovery, enzyme loading (wt% in the biocatalyst) and the physical properties, e.g. particle size and density, hydrophobicity and mechanical robustness of the immobilisate, i.e. the immobilised enzyme as a whole (enzyme + support). The choice of immobilisate is also strongly dependent on the reactor configuration used, e.g. stirred tank, fixed bed, fluidised bed, and the mode of downstream processing. Emphasis is placed on relatively recent developments, such as the use of novel supports such as mesoporous silicas, hydrogels, and smart polymers, and cross-linked enzyme aggregates (CLEAs).

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Occurrence of antibiotics and antibiotic resistance genes in hospital and urban wastewaters and their impact on the receiving river.

Sara Rodriguez-Mozaz, Sara Chamorro, Elisabet Marti … (2015)

Antibiotic resistance has become a major health concern; thus, there is a growing interest in exploring the occurrence of antibiotic resistance genes (ARGs) in the environment as well as the factors that contribute to their emergence. Aquatic ecosystems provide an ideal setting for the acquisition and spread of ARGs due to the continuous pollution by antimicrobial compounds derived from anthropogenic activities. We investigated, therefore, the pollution level of a broad range of antibiotics and ARGs released from hospital and urban wastewaters, their removal through a wastewater treatment plant (WWTP) and their presence in the receiving river. Several antimicrobial compounds were detected in all water samples collected. Among antibiotic families, fluoroquinolones were detected at the highest concentration, especially in hospital effluent samples. Although good removal efficiency by treatment processes was observed for several antimicrobial compounds, most antibiotics were still present in WWTP effluents. The results also revealed that copy numbers of ARGs, such as blaTEM (resistance to β-lactams), qnrS (reduced susceptibility to fluoroquinolones), ermB (resistance to macrolides), sulI (resistance to sulfonamides) and tetW (resistance to tetracyclines), were detected at the highest concentrations in hospital effluent and WWTP influent samples. Although there was a significant reduction in copy numbers of these ARGs in WWTP effluent samples, this reduction was not uniform across analyzed ARGs. Relative concentration of ermB and tetW genes decreased as a result of wastewater treatment, whereas increased in the case of blaTEM, sulI and qnrS genes. The incomplete removal of antibiotics and ARGs in WWTP severely affected the receiving river, where both types of emerging pollutants were found at higher concentration in downstream waters than in samples collected upstream from the discharge point. Taken together, our findings demonstrate a widespread occurrence of antibiotics and ARGs in urban and hospital wastewater and how these effluents, even after treatment, contribute to the spread of these emerging pollutants in the aquatic environment.

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Author and article information

Journal

Title: Water Science and Technology

Publisher: IWA Publishing

ISSN (Print): 0273-1223

ISSN (Electronic): 1996-9732

Publication date Created: April 01 2022

Publication date Created: March 22 2022

Publication date (Print): April 01 2022

Publication date (Electronic): March 22 2022

Volume: 85

Issue: 7

Pages: 2189-2207

Affiliations

[1 ]Department of Environmental Health Engineering, School of Public Health, Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

[2 ]Social Development & Health Promotion Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

[3 ]Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

[4 ]Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

Article

DOI: 10.2166/wst.2022.098

SO-VID: e0133063-44dc-47ed-9753-33f0c4e041a7

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http://creativecommons.org/licenses/by/4.0/

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Immobilization of IMP-1 metallo-beta-lactamase on Fe3O4@SiO2 as nanobiocatalyst for degradation of beta-lactam antibiotics in wastewater

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UCL: UN SDG 01 No Poverty

Most cited references 69

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

Enzyme immobilisation in biocatalysis: why, what and how.

Occurrence of antibiotics and antibiotic resistance genes in hospital and urban wastewaters and their impact on the receiving river.

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