Attenuating post-injury neuroendocrine stress abrogates persistent injury-associated
anemia. Our objective was to examine the mechanisms by which propranolol and clonidine
modulate this process. We hypothesized that propranolol and clonidine would decrease
bone marrow expression of high-mobility group box-1 (HMGB1) and increase expression
of stem cell factor (SCF) and B-cell lymphoma-extra large (Bcl-xL). Male Sprague-Dawley
rats were allocated to naïve control, lung contusion followed by hemorrhagic shock
(LCHS), or LCHS plus daily chronic restraint stress (LCHS/CS) ±propranolol, ±clonidine.
Day seven bone marrow expression of HMGB1, SCF, and Bcl-xL was assessed by polymerase
chain reaction. Following LCHS, HMGB1 was decreased by propranolol (49% decrease,
p=0.012) and clonidine (54% decrease, p<0.010). SCF was decreased following LCHS/CS,
and was increased by propranolol (629% increase, p<0.001) and clonidine (468% increase,
p<0.001). Bcl-xL was decreased following LCHS/CS, and was increased by propranolol
(59% increase, p=0.006) and clonidine (77% increase, p<0.001). Following severe trauma,
propranolol and clonidine abrogate persistent injury-associated anemia by modulating
bone marrow cytokines, favoring effective erythropoiesis. Attenuating the post-injury
neuroendocrine stress response with propranolol and clonidine has demonstrated efficacy
in abrogating persistent injury-associated anemia. Sprague-Dawley rats were subjected
to blunt trauma, hemorrhagic shock, and chronic restraint stress, with and without
propranolol and clonidine administration immediately following resuscitation. Attenuating
the neuroendocrine stress response with propranolol and clonidine modulated the bone
marrow cytokine response to severe trauma and chronic stress, favoring effective erythropoiesis.