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      Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae.

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          Abstract

          Extended-spectrum beta-lactamases (ESBLs) are often mediated by (bla-)SHV, (bla)TEM and (bla)CTX-M genes in Enterobacteriaceae and other Gram-negative bacteria. Numerous molecular typing methods, including PCR-based assays, have been developed for their identification. To reduce the number of PCR amplifications needed we have developed a multiplex PCR assay which detects and discriminates between (bla-)SHV, (bla)TEM and (bla)CTX-M PCR amplicons of 747, 445 and 593 bp, respectively. This multiplex PCR assay allowed the identification of (bla-)SHV, (bla)TEM and (bla)CTX-M genes in a series of clinical isolates of Enterobacteriaceae with previously characterised ESBL phenotype. The presence of (bla)SHV, (bla)TEM and (bla)CTX-M genes was confirmed by partial DNA sequence analysis. Apparently, the universal well-established CTX-M primer pair used here to reveal plasmid-encoded (bla)CTX-M genes would also amplify the chromosomally located K-1 enzyme gene in all Klebsiella oxytoca strains included in the study.

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          Author and article information

          Journal
          APMIS
          APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
          Wiley
          0903-4641
          0903-4641
          Dec 2007
          : 115
          : 12
          Affiliations
          [1 ] Division of Clinical Microbiology, University Hospital Linköping, Linköping, Sweden.
          Article
          APMapm_722.xml
          10.1111/j.1600-0463.2007.00722.x
          18184411
          dca2f062-4d42-44cf-add1-69ece962e9ff
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