SSR‐based DNA fingerprinting of fruit crops

Abstract

The DNA fingerprinting of fruit crops, based on DNA microsatellite markers that are considered to be the key markers for the molecular analysis of germplasm collections, is reviewed. Simple sequence repeats (SSRs) remain the markers of choice for fingerprinting in humans, animals, plants, and other living organisms. This review, that considers 44 fruit species, provides a set of markers that are suitable for profiling accessions and that should make the databases produced in different laboratories more comparable. Every effort has been made to select SSR markers that are robust and easily scorable considering that such analyses are sometimes used in legal cases. The review first describes the basic protocols, procedures, and methods of data analyses; it then describes the fingerprinting of individual species or groups of species, providing a set of SSR markers and appropriate guidance based on the revised literature and on the authors’ experience.

Core Ideas

• DNA‐based fingerprinting is a modern molecular approach to characterize germplasm resources.

• SSR (simple sequence repeats) or microsatellites are nowadays the molecular markers of election for genotyping.

• This review reports protocols and markers selected for fingerprinting 44 fruit crop species.