3
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell’s response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells.

          Abstract

          In all living organisms, cells must resist mechanical stress to survive. This study of the model plant Arabidopsis reveals that the candidate cell wall mechanoreceptor FERONIA and microtubules independently contribute to this mechanical feedback.

          Related collections

          Most cited references58

          • Record: found
          • Abstract: not found
          • Article: not found

          FactoMineR: AnRPackage for Multivariate Analysis

            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Growth of the plant cell wall.

            Plant cells encase themselves within a complex polysaccharide wall, which constitutes the raw material that is used to manufacture textiles, paper, lumber, films, thickeners and other products. The plant cell wall is also the primary source of cellulose, the most abundant and useful biopolymer on the Earth. The cell wall not only strengthens the plant body, but also has key roles in plant growth, cell differentiation, intercellular communication, water movement and defence. Recent discoveries have uncovered how plant cells synthesize wall polysaccharides, assemble them into a strong fibrous network and regulate wall expansion during cell growth.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              FibrilTool, an ImageJ plug-in to quantify fibrillar structures in raw microscopy images.

              Cell biology heavily relies on the behavior of fibrillar structures, such as the cytoskeleton, yet the analysis of their behavior in tissues often remains qualitative. Image analysis tools have been developed to quantify this behavior, but they often involve an image pre-processing stage that may bias the output and/or they require specific software. Here we describe FibrilTool, an ImageJ plug-in based on the concept of nematic tensor, which can provide a quantitative description of the anisotropy of fiber arrays and their average orientation in cells, directly from raw images obtained by any form of microscopy. FibrilTool has been validated on microtubules, actin and cellulose microfibrils, but it may also help analyze other fibrillar structures, such as collagen, or the texture of various materials. The tool is ImageJ-based, and it is therefore freely accessible to the scientific community and does not require specific computational setup. The tool provides the average orientation and anisotropy of fiber arrays in a given region of interest (ROI) in a few seconds.
                Bookmark

                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: MethodologyRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
                Role: Data curationRole: Formal analysisRole: Methodology
                Role: Data curationRole: Formal analysis
                Role: Data curationRole: Formal analysis
                Role: Data curationRole: Formal analysis
                Role: Data curationRole: Formal analysis
                Role: Data curationRole: Formal analysis
                Role: Data curationRole: Formal analysis
                Role: ConceptualizationRole: Funding acquisitionRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
                Role: ConceptualizationRole: Formal analysisRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
                Role: Academic Editor
                Journal
                PLoS Biol
                PLoS Biol
                plos
                PLoS Biology
                Public Library of Science (San Francisco, CA USA )
                1544-9173
                1545-7885
                12 November 2021
                November 2021
                12 November 2021
                : 19
                : 11
                : e3001454
                Affiliations
                [001] Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, UCB Lyon 1, ENS de Lyon, INRAE, CNRS, Lyon, France
                UCSD, UNITED STATES
                Author notes

                The authors have declared that no competing interests exist.

                [¤]

                Current address: Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden

                Author information
                https://orcid.org/0000-0002-0633-4242
                https://orcid.org/0000-0002-6873-9341
                https://orcid.org/0000-0001-6906-6620
                https://orcid.org/0000-0003-3643-3978
                Article
                PBIOLOGY-D-21-01644
                10.1371/journal.pbio.3001454
                8612563
                34767544
                d83d4dde-f27f-4e98-9a42-8aad72a27cea
                © 2021 Malivert et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 21 June 2021
                : 22 October 2021
                Page count
                Figures: 5, Tables: 0, Pages: 22
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/100011199, fp7 ideas: european research council;
                Award ID: ERC-2013-CoG-615739
                Award Recipient :
                This work was supported by the European Research Council (ERC-2013-CoG-615739 ’MechanoDevo’ to O.H). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Research Article
                Biology and Life Sciences
                Cell Biology
                Cellular Structures and Organelles
                Cytoskeleton
                Microtubules
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Leaves
                Pavement Cells
                Biology and Life Sciences
                Cell Biology
                Cellular Types
                Plant Cells
                Pavement Cells
                Biology and Life Sciences
                Cell Biology
                Plant Cell Biology
                Plant Cells
                Pavement Cells
                Biology and Life Sciences
                Plant Science
                Plant Cell Biology
                Plant Cells
                Pavement Cells
                Biology and Life Sciences
                Cell Biology
                Cell Processes
                Cellular Stress Responses
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Fruit and Seed Anatomy
                Plant Embryo Anatomy
                Hypocotyl
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Plant Embryo Anatomy
                Hypocotyl
                Biology and Life Sciences
                Developmental Biology
                Embryogenesis
                Plant Embryogenesis
                Plant Embryo Anatomy
                Hypocotyl
                Biology and Life Sciences
                Developmental Biology
                Plant Growth and Development
                Plant Development
                Plant Embryogenesis
                Plant Embryo Anatomy
                Hypocotyl
                Biology and Life Sciences
                Plant Science
                Plant Growth and Development
                Plant Development
                Plant Embryogenesis
                Plant Embryo Anatomy
                Hypocotyl
                Biology and Life Sciences
                Organisms
                Eukaryota
                Plants
                Seedlings
                Physical Sciences
                Physics
                Classical Mechanics
                Mechanical Stress
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Fruit and Seed Anatomy
                Plant Embryo Anatomy
                Plant Cotyledon
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Plant Embryo Anatomy
                Plant Cotyledon
                Biology and Life Sciences
                Developmental Biology
                Embryogenesis
                Plant Embryogenesis
                Plant Embryo Anatomy
                Plant Cotyledon
                Biology and Life Sciences
                Developmental Biology
                Plant Growth and Development
                Plant Development
                Plant Embryogenesis
                Plant Embryo Anatomy
                Plant Cotyledon
                Biology and Life Sciences
                Plant Science
                Plant Growth and Development
                Plant Development
                Plant Embryogenesis
                Plant Embryo Anatomy
                Plant Cotyledon
                Physical Sciences
                Chemistry
                Chemical Compounds
                Organic Compounds
                Cellulose
                Physical Sciences
                Chemistry
                Organic Chemistry
                Organic Compounds
                Cellulose
                Custom metadata
                vor-update-to-uncorrected-proof
                2021-11-24
                All relevant data are within the paper and its Supporting Information files.

                Life sciences
                Life sciences

                Comments

                Comment on this article