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      Zinc oxide nano-particles as sealer in endodontics and its sealing ability

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          Abstract

          Aims:

          The aim of this study was to evaluate the sealing ability of new experimental nano-ZOE-based sealer.

          Settings and Design:

          Three types of nano-ZOE-based sealer (calcined at different temperatures of 500, 600 and 700°C) with two other commercially available sealers (AH26 and micro-sized zinc oxide eugenol sealer) were used.

          Materials and Methods:

          Zinc oxide nano-particles were synthesized by a modified sol-gel method. The structure and morphology of the prepared powders were characterized using x-ray diffraction (XRD) and transmission electron microscopy (TEM) techniques. The instrumented canals of 60 single-rooted teeth were divided into five groups ( n = 10), with the remaining ten used as controls. The canals were filled with gutta-percha using one of the materials mentioned above as sealer. After 3, 45 and 90 days, the samples were connected to a fluid filtration system.

          Statistical Analysis Used:

          The data were analyzed using Student's t-test.

          Results:

          The XRD patterns and TEM images revealed that all the synthesized powders had hexagonal wurtzite structures with an average particle size of about 30-60 nm at different calcination temperatures. Microleakage in AH26 groups was significantly more than that in three groups of ZnO nano-particles at all the three evaluation intervals. Apical microleakage of ZnO micro-powders was significantly more than that of all the materials, but the sealing ability of ZnO nano-powder sealers did not differ significantly.

          Conclusion:

          The results of this study showed that the synthesized ZnO nano-powder sealers are suitable for use as a nano-sealer in root canal therapy to prevent leakage; however, further studies should be carried out to verify their safety.

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          Most cited references26

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          Thin-film transistor fabricated in single-crystalline transparent oxide semiconductor.

          We report the fabrication of transparent field-effect transistors using a single-crystalline thin-film transparent oxide semiconductor, InGaO3(ZnO)5, as an electron channel and amorphous hafnium oxide as a gate insulator. The device exhibits an on-to-off current ratio of approximately 106 and a field-effect mobility of approximately 80 square centimeters per volt per second at room temperature, with operation insensitive to visible light irradiation. The result provides a step toward the realization of transparent electronics for next-generation optoelectronics.
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            Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells.

            The specific role of size scale, surface capping, and aspect ratio of zinc oxide (ZnO) particles on toxicity toward prokaryotic and eukaryotic cells was investigated. ZnO nano and microparticles of controlled size and morphology were synthesized by wet chemical methods. Cytotoxicity toward mammalian cells was studied using a human osteoblast cancer cell line and antibacterial activity using Gram-negative bacteria (Escherichia coli) as well as using Gram-positive bacteria (Staphylococcus aureus). Scanning electron microscopy (SEM) was conducted to characterize any visual features of the biocidal action of ZnO. We observed that antibacterial activity increased with reduction in particle size. Toxicity toward the human cancer cell line was considerably higher than previously observed by other researchers on the corresponding primary cells, suggesting selective toxicity of the ZnO to cancer cells. Surface capping was also found to profoundly influence the toxicity of ZnO nanoparticles toward the cancer cell line, with the toxicity of starch-capped ZnO being the lowest. Our results are found to be consistent with a membrane-related mechanism for nanoparticle toxicity toward microbes.
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              Preparation and properties of nano-sized calcium fluoride for dental applications.

              The aim of the present study was to prepare nano-sized calcium fluoride (CaF(2)) that could be used as a labile F reservoir for more effective F regimens and as an agent for use in the reduction of dentin permeability. Nano-sized CaF(2) powders were prepared using a spray-drying system with a two-liquid nozzle. The properties of the nano-CaF(2) were studied and the effectiveness of a fluoride (F) rinse with nano-CaF(2) as the F source was evaluated. The thermodynamic solubility product of the nano-CaF(2) solution was determined by equilibrating the nanosample in solutions presaturated with respect to macro-CaF(2). Reactivity of the nano-CaF(2) was assessed by its reaction with dicalcium phosphate dehydrate (DCPD). F deposition by 13.2 mmol/L F rinse with the nano-CaF(2) as the F source was determined using a previously published in vitro model. X-ray diffraction (XRD) analysis showed pattern of low crystalline CaF(2). BET measurements showed that the nano-CaF(2) had a surface area of 46.3m(2)/g, corresponding to a particle size of 41nm. Transmission electron microscopy (TEM) examinations indicated that the nano-CaF(2) contained clusters comprising particles of (10-15) nm in size. The nano-CaF(2) displayed much higher solubility and reactivity than its macro-counterpart. The CaF(2) ion activity product (IAP) of the solution in equilibrium with the nano-CaF(2) was (1.52+/-0.05)x10(-10), which was nearly four times greater than the K(sp) (3.9 x 10(-11)) for CaF(2). The reaction of DCPD with nano-CaF(2) resulted in more F-containing apatitic materials compared to the reaction with macro-CaF(2). The F deposition by the nano-CaF(2) rinse was (2.2+/-0.3)mug/cm(2) (n=5), which was significantly (p<0.001) greater than that ((0.31+/-0.06)mug/cm(2)) produced by the NaF solution. The nano-CaF(2) can be used as an effective anticaries agent in increasing the labile F concentration in oral fluid and thus enhance the tooth remineralization. It can also be very useful in the treatment for the reduction of dentin permeability.
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                Author and article information

                Journal
                Contemp Clin Dent
                Contemp Clin Dent
                CCD
                Contemporary Clinical Dentistry
                Medknow Publications & Media Pvt Ltd (India )
                0976-237X
                0976-2361
                Jan-Mar 2014
                : 5
                : 1
                : 20-24
                Affiliations
                [1] Dental Material Research Center, Department of Endodontics, School of Dentistry, Mashhad University of Medical Sciences, Iran
                [1 ] Dental Research Center, Department of Endodontics, School of Dentistry, Mashhad University of Medical Sciences (MUMS), Iran
                [2 ] Endodontist, Private Practice, Iran
                [3 ] General Practitioner, Iran
                Author notes
                Correspondence: Dr. Neda Naghavi, Dental Research Center, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran. E-mail: naghavin@ 123456mums.ac.ir
                Article
                CCD-5-20
                10.4103/0976-237X.128656
                4012111
                24808690
                d5ca0f05-6a73-4e1c-a359-f37760b5114f
                Copyright: © Contemporary Clinical Dentistry

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Categories
                Original Article

                Dentistry
                ah26,dental material,microleakage,nano-particle sealers,sealer,zinc oxide eugenol
                Dentistry
                ah26, dental material, microleakage, nano-particle sealers, sealer, zinc oxide eugenol

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