Antifungal-demelanizing properties and RAW264.7 macrophages stimulation of glucan sulfate from the mycelium of the mushroom <i>Ganoderma lucidum</i>

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Abstract

Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 μg/mL to produce 0.45 μM of nitric oxide. The GS-stimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.

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Author and article information

Contributors

Wan Abd Al Qadr Imad Wan-Mohtar: +601118903358 , +60379674178 , qadyr@um.edu.my , wanqadir1987@gmail.com

Journal

Journal ID (nlm-ta): Food Sci Biotechnol

Journal ID (iso-abbrev): Food Sci. Biotechnol

Title: Food Science and Biotechnology

Publisher: The Korean Society of Food Science and Technology (Seoul )

ISSN (Print): 1226-7708

ISSN (Electronic): 2092-6456

Publication date (Electronic): 28 February 2017

Publication date Collection: 2017

Volume: 26

Issue: 1

Pages: 159-165

Affiliations

[1 ] ISNI 0000 0001 2308 5949, GRID grid.10347.31, Institute of Biological Sciences, Faculty of Science, , University of Malaya, ; Kuala Lumpur, 50603 Malaysia

[2 ] ISNI 0000000121138138, GRID grid.11984.35, Fermentation Centre, Strathclyde Institute of Pharmacy and Biomedical Sciences, , University of Strathclyde, ; Glasgow, Scotland, U.K. G4 0RE UK

[3 ] ISNI 0000 0001 2166 9385, GRID grid.7149.b, Institute for Food Technology and Biochemistry, , University of Belgrade - Faculty of Agriculture, ; Nemanjina 6, 11080 Belgrade, Serbia

[4 ] ISNI 0000 0001 2231 800X, GRID grid.11142.37, Department of Food Technology, , Faculty of Food Science and Technology Universiti Putra Malaysia, ; Serdang, Malaysia

[5 ] ISNI 0000 0001 2308 5949, GRID grid.10347.31, Mushroom Research Centre, , University of Malaya, ; Kuala Lumpur, 50603 Malaysia

Article

Accession ID: PMC6049470 Pmcid ID: PMC6049470 Pmc-uid ID: 6049470 Publisher ID: 21

DOI: 10.1007/s10068-017-0021-6

PMC ID: 6049470

PubMed ID: 30263523

SO-VID: d4eab7ff-1668-44b3-947d-42d3f7a4f12e

History

Date received : 28 June 2016

Date revision received : 8 November 2016

Date accepted : 9 December 2016

Custom metadata

issue-copyright-statement © The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2017

Keywords: nitric oxide production,antifungal activity,demelanization, Ganoderma lucidum ,glucan sulfate

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Keywords: nitric oxide production, antifungal activity, demelanization, Ganoderma lucidum , glucan sulfate

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