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      Organogênese in vitro a partir de explante caulinar na regeneração de clones de Eucalyptus grandis W. Hill ex Maiden X E. urophylla S. T. Blake Translated title: In vitro regeneration from stem explants of Eucalyptus grandis x E. urophylla clones through organogenesis

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          Abstract

          Com o objetivo de testar a regeneração in vitro por organogênese a partir de explante caulinar de três clones híbridos de Eucalyptus grandis x Eucalyptus urophylla, foram avaliados os efeitos dos reguladores de crescimento TDZ [1-fenil-3-(1,2,3-tiadiazol-5-il)uréia], BAP (6-benzilaminopurina) e ANA (ácido naftalenoacético). De modo geral, pôde-se observar resposta diferenciada dos clones quanto a intensidade, textura, coloração e grau de oxidação dos calos, em função dos tratamentos com os reguladores de crescimento. Os melhores resultados de calejamento foram dos tratamentos com a combinação dos reguladores de crescimento TDZ (0,5 mg L-1) e ANA (0,1 mg L-1), obtendo-se 100% de calejamento no explante caulinar. Houve a formação de estruturas nodulares compactas, principalmente na extremidade dos explantes caulinares, sendo essas regiões responsáveis pela regeneração de gemas adventícias. Em relação à regeneração, a melhor resposta foi obtida com 1,0 mg L-1 BAP.

          Translated abstract

          With the objective of testing in vitro regeneration through organogenesis from stem explants of three hybrid clones of Eucalyptus grandis x Eucalyptus urophylla, the effects of the growth regulators TDZ [1-phenil-3-(1, 2, 3-thiadiazol-5-il)urea], BAP (6-benzilaminopurine) and NAA (Naphthalene acetic acid) were evaluated. In general, a differentiated clone response in relation to intensity, texture, color and oxidation degree of calli was observed due to growth regulator treatments. The best results in callus formation were recorded for the treatments with the combination TDZ (0,5 mg L-1) and ANA (0,1 mg L-1), achieving 100% of callus formation from the stem explants. Compact node structures appeared mainly in the extremities of the stem explants, being those areas responsible for the regeneration of adventitious buds. The best regeneration response was obtained with 1,0 mg L-1 BAP.

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          Most cited references25

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          Thidiazuron: A potent regulator ofin vitro plant morphogenesis

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            The use of thidiazuron in tissue culture

            Chin-Yi Lu (1993)
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              Auxin-cytokinin interactions in higher plants: old problems and new tools.

              The plant hormones auxin and cytokinin interact in a complex manner to control many aspects of growth and differentiation. Recent advances in understanding their metabolism and the cell cycle are now helping to clarify the mechanisms by which these hormones act together to control various physiological and developmental responses. Molecular and genetic tools are being used to reveal interactions between auxin and cytokinin at multiple levels, including mutual regulation of active hormone availability, input into multiple signal transduction pathways, alteration of gene expression, post-translational modifications and direct modulation of enzyme activity.
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                Author and article information

                Journal
                rarv
                Revista Árvore
                Rev. Árvore
                Sociedade de Investigações Florestais (Viçosa, MG, Brazil )
                0100-6762
                1806-9088
                October 2004
                : 28
                : 5
                : 643-653
                Affiliations
                [01] Viçosa MG orgnameUniversidade Federal de Viçosa orgdiv1Departamento de Engenharia Florestal xavier@ 123456ufv.br
                [02] Viçosa MG orgnameUniversidade Federal de Viçosa orgdiv1Departamento de Biologia Vegetal wotoni@ 123456ufv.br
                Article
                S0100-67622004000500003 S0100-6762(04)02800503
                d422fd8a-94fa-4776-bb3c-0d5060580f71

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 10 August 2004
                : 30 April 2003
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 25, Pages: 11
                Product

                SciELO Brazil


                Micropropagação,microprogation,reguladores de crescimento e propagação in vitro,growth regulator.and in vitro propagation

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