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Abstract
Large yellow croaker (Pseudosciaena crocea) is one of the most important marine cultured
fish in China. Acidic extracts of five tissues of large yellow croaker showed strong
anti-Vibrio alginolyticus activity. Acidic extract of head kidney tissue was subjected
to heat-treatment in boiling water, and solid-phase extraction on Sep-Pak C(18) cartridge.
It was found that the antibacterial substances were heat stable, and 20% acetonitrile
effluent exhibited strong antibacterial activity. Active extract was further applied
to Sephadex G-25 gel permeation chromatography and StableBond C(18) RP-HPLC. An antibacterial
peptide with a single peak was obtained. The results of amino acid sequencing and
MALDI-TOF MS suggested that the peptide was RCRFCCRCCPRMRGCGICCRF with an observed
molecular mass of 2523.2 Da. BLAST searching suggested that the purified antibacterial
peptide was the mature peptide section of the hepcidin preproprotein presumed from
cDNA of large yellow croaker, thus designated hepcidin-Pl. Hepcidin-P1 exhibited strong
antibacterial activity against four marine vibrios.