Maternal transfer of immunity in scallop Chlamys farreri and its trans-generational immune protection to offspring against bacterial challenge.

Abstract

Maternal immunity plays a crucial role in protecting the offspring at early stages of life and contributes a trans-generational effect on the offspring's phenotype. In the present study, maternal transfer of immunity and its trans-generational effect on offspring in scallop Chlamys farreri were investigated. The proteins including CfLGBP, CfLBP/BPI, CfLYZ and CfCu/Zn-SOD existed in the scallop eggs with high level while CfLec-3 was not detected. In contrast, the mRNA levels of these proteins were extremely low except that of CfCu/Zn-SOD. The protein extracts of scallop eggs exhibited remarkable agglutination activity and bactericidal effect against gram-negative bacteria Escherichia coli and Vibro anguillarum, and fungi Pichia pastoris. When the maternal scallops were stimulated with heat-killed V. anguillarum, the mRNA levels of CfLBP/BPI and CfLYZ in their offspring were expressed significant higher in D-shaped larvae. Furthermore, the protein levels of CfLBP/BPI and CfCu/Zn-SOD in the offspring of maternal immune stimulation group were higher than that of control at almost all the developmental stages, while the level of CfLec-3 and CfLYZ was higher than that of control just in eggs or trochophore, respectively. A significant enhancement of Cu/Zn-SOD and antibacterial activities was also observed in eggs, 4-cell embryos and trochophore of offspring from immune stimulated mother scallops. Moreover, the mortality of offspring from the immune stimulated mother scallops was significantly lower than that of control after bacterial challenge, especially in trochophore. The results indicated that scallop eggs or embryos received maternal derived immune competence to defense against the invading pathogens, and the maternal scallops received an immune stimulation endowed their offspring with a trans-generational immune capability to protect them against infections effectively.