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      Biodegradation of Low-Density Polyethylene—LDPE by the Lepidopteran Galleria Mellonella Reusing Beekeeping Waste

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          Abstract

          Plastic pollution is one of the most serious environmental problems of this century because most plastics are single-use, and once their useful life is over, they become pollutants, since their decomposition takes approximately 100–400 years. The objective of this research is to evaluate the efficacy of low-density polyethylene (LDPE) biodegradation by G. mellonella in the district of Pangoa, Junín, Peru. For the development of the study, the G. mellonella was conditioned in three groups of beekeeping residues (beeswax, balanced diet, and wheat bran); after the conditioning stage, the biodegradation treatment was developed, which consisted of placing the G. mellonella in terrariums with the LDPE, the treatments were carried out at three different times (24, 36, and 48 h). To evaluate the efficacy of biodegradation, two analyses were taken into account: the Raman analysis of the low-density polyethylene samples and the weight reduction of the treated LDPE. The results of the Raman analysis indicated that the best treatment was the one applied with G. mellonella conditioned with beeswax, obtaining a wavelength intensity of 0.45 μ.a., while the weight reduction of the LDPE indicated that the best results were given at 36 h and conditioned with beeswax with a reduction of 236.3 mg. In conclusion, the use of G. mellonella for the biodegradation of low-density polyethylene is effective when it is conditioned with beeswax and the treatment is carried out at 36 h.

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          Production, use, and fate of all plastics ever made

          We present the first ever global account of the production, use, and end-of-life fate of all plastics ever made by humankind.
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            Evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms.

            Polyethylene (PE) has been considered nonbiodegradable for decades. Although the biodegradation of PE by bacterial cultures has been occasionally described, valid evidence of PE biodegradation has remained limited in the literature. We found that waxworms, or Indian mealmoths (the larvae of Plodia interpunctella), were capable of chewing and eating PE films. Two bacterial strains capable of degrading PE were isolated from this worm's gut, Enterobacter asburiae YT1 and Bacillus sp. YP1. Over a 28-day incubation period of the two strains on PE films, viable biofilms formed, and the PE films' hydrophobicity decreased. Obvious damage, including pits and cavities (0.3-0.4 μm in depth), was observed on the surfaces of the PE films using scanning electron microscopy (SEM) and atomic force microscopy (AFM). The formation of carbonyl groups was verified using X-ray photoelectron spectroscopy (XPS) and microattenuated total reflectance/Fourier transform infrared (micro-ATR/FTIR) imaging microscope. Suspension cultures of YT1 and YP1 (10(8) cells/mL) were able to degrade approximately 6.1 ± 0.3% and 10.7 ± 0.2% of the PE films (100 mg), respectively, over a 60-day incubation period. The molecular weights of the residual PE films were lower, and the release of 12 water-soluble daughter products was also detected. The results demonstrated the presence of PE-degrading bacteria in the guts of waxworms and provided promising evidence for the biodegradation of PE in the environment.
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              Review on the current status of polymer degradation: a microbial approach

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                Author and article information

                Contributors
                Journal
                Front Bioeng Biotechnol
                Front Bioeng Biotechnol
                Front. Bioeng. Biotechnol.
                Frontiers in Bioengineering and Biotechnology
                Frontiers Media S.A.
                2296-4185
                09 September 2022
                2022
                : 10
                : 915331
                Affiliations
                [1] 1 Escuela Profesional de Ingeniería Ambiental , Facultad de Ingeniería y Arquitectura , Universidad Peruana Unión , Lima, Perú
                [2] 2 Escuela Profesional de Ingeniería Ambiental , Facultad de Ingeniería y Arquitectura , Universidad Peruana Unión , Juliaca, Perú
                [3] 3 Escuela UPG Ingeniería y Arquitectura , Escuela de Posgrado , Universidad Peruana Unión , Lima, Perú
                Author notes

                Edited by: Zhanyong Wang, Shenyang Agricultural University, China

                Reviewed by: Jingjing Zhao, Liaoning Shihua University, China

                Vinay Mohan Pathak, University of Delhi, India

                *Correspondence: Betty Ricce, bettyricce@ 123456upeu.edu.pe ; Orlando Poma, opoma@ 123456upeu.edu.pe

                This article was submitted to Bioprocess Engineering, a section of the journal Frontiers in Bioengineering and Biotechnology

                Article
                915331
                10.3389/fbioe.2022.915331
                9535396
                ce42c2c0-d113-429c-8081-cb364d8a63b2
                Copyright © 2022 Poma, Ricce, Beraún, Perez Carpio, Fernandez and Soria.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 07 April 2022
                : 24 May 2022
                Categories
                Bioengineering and Biotechnology
                Original Research

                pollution,plastic,galleria mellonella,ldpe,biodegradation
                pollution, plastic, galleria mellonella, ldpe, biodegradation

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