Antibacterial Activity of Green Synthesized Silver Nanoparticles Using <i>Lawsonia inermis</i> Against Common Pathogens from Urinary Tract Infection

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Abstract

New and creative methodologies for the fabrication of silver nanoparticles (Ag-NPs), which are exploited in a wide range of consumer items, are of significant interest. Hence, this research emphasizes the biological approach of Ag-NPs through Egyptian henna leaves ( Lawsonia inermis Linn.) extracts and analysis of the prepared Ag-NPs. Plant extract components were identified by gas chromatography mass spectrometry (GC-mass). The analyses of prepared Ag-NPs were carried out through UV–visible (UV–Vis), X-ray diffraction (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and Fourier transform infrared (FTIR) analysis. UV–Vis reveals that Ag-NPs have a maximum peak at 460 nm in visible light. Structural characterization recorded peaks that corresponded to Bragg’s diffractions for silver nano-crystal, with average crystallite sizes varying from 28 to 60 nm. Antibacterial activities of Ag-NPs were examined, and it is observed that all microorganisms are very sensitive to biologically synthesized Ag-NPs.

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Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: an Overview

Salem S. Salem, Amr Fouda (2020)

The green synthesis of nanoparticles (NPs) using living cells is a promising and novelty tool in bionanotechnology. Chemical and physical methods are used to synthesize NPs; however, biological methods are preferred due to its eco-friendly, clean, safe, cost-effective, easy, and effective sources for high productivity and purity. High pressure or temperature is not required for the green synthesis of NPs, and the use of toxic and hazardous substances and the addition of external reducing, stabilizing, or capping agents are avoided. Intra- or extracellular biosynthesis of NPs can be achieved by numerous biological entities including bacteria, fungi, yeast, algae, actinomycetes, and plant extracts. Recently, numerous methods are used to increase the productivity of nanoparticles with variable size, shape, and stability. The different mechanical, optical, magnetic, and chemical properties of NPs have been related to their shape, size, surface charge, and surface area. Detection and characterization of biosynthesized NPs are conducted using different techniques such as UV-vis spectroscopy, FT-IR, TEM, SEM, AFM, DLS, XRD, zeta potential analyses, etc. NPs synthesized by the green approach can be incorporated into different biotechnological fields as antimicrobial, antitumor, and antioxidant agents; as a control for phytopathogens; and as bioremediative factors, and they are also used in the food and textile industries, in smart agriculture, and in wastewater treatment. This review will address biological entities that can be used for the green synthesis of NPs and their prospects for biotechnological applications.

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Nano-Strategies to Fight Multidrug Resistant Bacteria—“A Battle of the Titans”

Pedro V. Baptista, Matthew P. McCusker, Andreia A. Carvalho … (2018)

Infectious diseases remain one of the leading causes of morbidity and mortality worldwide. The WHO and CDC have expressed serious concern regarding the continued increase in the development of multidrug resistance among bacteria. Therefore, the antibiotic resistance crisis is one of the most pressing issues in global public health. Associated with the rise in antibiotic resistance is the lack of new antimicrobials. This has triggered initiatives worldwide to develop novel and more effective antimicrobial compounds as well as to develop novel delivery and targeting strategies. Bacteria have developed many ways by which they become resistant to antimicrobials. Among those are enzyme inactivation, decreased cell permeability, target protection, target overproduction, altered target site/enzyme, increased efflux due to over-expression of efflux pumps, among others. Other more complex phenotypes, such as biofilm formation and quorum sensing do not appear as a result of the exposure of bacteria to antibiotics although, it is known that biofilm formation can be induced by antibiotics. These phenotypes are related to tolerance to antibiotics in bacteria. Different strategies, such as the use of nanostructured materials, are being developed to overcome these and other types of resistance. Nanostructured materials can be used to convey antimicrobials, to assist in the delivery of novel drugs or ultimately, possess antimicrobial activity by themselves. Additionally, nanoparticles (e.g., metallic, organic, carbon nanotubes, etc.) may circumvent drug resistance mechanisms in bacteria and, associated with their antimicrobial potential, inhibit biofilm formation or other important processes. Other strategies, including the combined use of plant-based antimicrobials and nanoparticles to overcome toxicity issues, are also being investigated. Coupling nanoparticles and natural-based antimicrobials (or other repurposed compounds) to inhibit the activity of bacterial efflux pumps; formation of biofilms; interference of quorum sensing; and possibly plasmid curing, are just some of the strategies to combat multidrug resistant bacteria. However, the use of nanoparticles still presents a challenge to therapy and much more research is needed in order to overcome this. In this review, we will summarize the current research on nanoparticles and other nanomaterials and how these are or can be applied in the future to fight multidrug resistant bacteria.

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Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates.

S Arokiyaraj, Jaya P Gnanaprakasam, Joann Emmanuel … (2013)

Green synthesis of metallic silver nanoparticles has attracted nowadays and alternative to physical and chemical approaches. In the present study, silver nanoparticles (AgNPs) were synthesized from leaf extract of Mimusops elengi, L. at room temperature. Formation of stable AgNPs at 1mM concentrations of silver nitrate (AgNO3) typically gave spherical shape particles with diameter range from 55 to 83nm. The kinetic properties of particle formation were proportional to the effect of concentration of AgNO3 solution. In order to identify the compounds responsible for the bioreduction of Ag(+) ion and the stabilization of AgNPs produced, the functional group present in Mimusops elengi, L. leaf extract was investigated using FTIR. The formation of nanoparticle was confirmed using the surface plasmon resonance band shown in UV-vis spectrophotometer. The topography and morphology of the particles were determined using scanning electron microscopy. The crystalline nature of nanoparticles was confirmed from the XRD pattern. Furthermore these green synthesized AgNPs were found to show higher antimicrobial efficacy against multi drug resistant clinical isolates. Copyright © 2013 Elsevier B.V. All rights reserved.

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Author and article information

Contributors

Salem S. Salem:

ORCID: http://orcid.org/0000-0003-2898-6708

salemsalahsalem@azhar.edu.eg

Journal

Journal ID (nlm-ta): Appl Biochem Biotechnol

Journal ID (iso-abbrev): Appl Biochem Biotechnol

Title: Applied Biochemistry and Biotechnology

Publisher: Springer US (New York )

ISSN (Print): 0273-2289

ISSN (Electronic): 1559-0291

Publication date (Electronic): 26 April 2023

Publication date PMC-release: 26 April 2023

Publication date (Print): 2024

Volume: 196

Issue: 1

Pages: 85-98

Affiliations

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, ( https://ror.org/05fnp1145) Nasr City, 11884 Cairo Egypt

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Salem S. Salem http://orcid.org/0000-0003-2898-6708

Article

Publisher ID: 4482

DOI: 10.1007/s12010-023-04482-1

PMC ID: 10794286

PubMed ID: 37099124

SO-VID: c8f6016a-53e3-4f6d-a398-e26e3dbebe32

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

History

Date accepted : 11 April 2023

Funding

Funded by: Al-Azhar University

Open Access :

Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).

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ScienceOpen disciplines: Biochemistry

Keywords: silver nanoparticles,lawsonia inermis linn.,antibacterial activity,urinary tract infection

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ScienceOpen disciplines: Biochemistry

Keywords: silver nanoparticles, lawsonia inermis linn., antibacterial activity, urinary tract infection

Antibacterial Activity of Green Synthesized Silver Nanoparticles Using Lawsonia inermis Against Common Pathogens from Urinary Tract Infection

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Photoluminescent Nanomaterials

Most cited references 50

Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: an Overview

Nano-Strategies to Fight Multidrug Resistant Bacteria—“A Battle of the Titans”

Green synthesis of silver nanoparticles from leaf extract of Mimusops elengi, Linn. for enhanced antibacterial activity against multi drug resistant clinical isolates.

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