Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings

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Abstract

This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric point of 5.84 and the other has lowest LOX activity with an isoelectric point of 5.52. The purified LOX has molecular mass of approximately 97 kDa and showed high activity with linoleic acid than linolenic acid and arachidonic acid. The optimal activity of LOX was observed at pH 6.5 and temperature 35 °C. Far-UV circular dichroism (CD) studies revealed that the purified mung bean LOX possess secondary structural elements with significant α-helix and β-strands. Further, the secondary structure of mung bean LOX was stable up to 60 °C at pH 6.5. Biophysical and chemical properties of the mung bean LOX are similar to the other legume LOXs and may be considered as type-1 LOX.

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The lipoxygenase pathway.

Ivo Feussner, Claus Wasternack (2002)

Lipid peroxidation is common to all biological systems, both appearing in developmentally and environmentally regulated processes of plants. The hydroperoxy polyunsaturated fatty acids, synthesized by the action of various highly specialized forms of lipoxygenases, are substrates of at least seven different enzyme families. Signaling compounds such as jasmonates, antimicrobial and antifungal compounds such as leaf aldehydes or divinyl ethers, and a plant-specific blend of volatiles including leaf alcohols are among the numerous products. Cloning of many lipoxygenases and other key enzymes within the lipoxygenase pathway, as well as analyses by reverse genetic and metabolic profiling, revealed new reactions and the first hints of enzyme mechanisms, multiple functions, and regulation. These aspects are reviewed with respect to activation of this pathway as an initial step in the interaction of plants with pathogens, insects, or abiotic stress and at distinct stages of development.

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First off the mark: early seed germination.

Karin Weitbrecht, Kerstin Müller, Gerhard Leubner-Metzger (2011)

Most plant seeds are dispersed in a dry, mature state. If these seeds are non-dormant and the environmental conditions are favourable, they will pass through the complex process of germination. In this review, recent progress made with state-of-the-art techniques including genome-wide gene expression analyses that provided deeper insight into the early phase of seed germination, which includes imbibition and the subsequent plateau phase of water uptake in which metabolism is reactivated, is summarized. The physiological state of a seed is determined, at least in part, by the stored mRNAs that are translated upon imbibition. Very early upon imbibition massive transcriptome changes occur, which are regulated by ambient temperature, light conditions, and plant hormones. The hormones abscisic acid and gibberellins play a major role in regulating early seed germination. The early germination phase of Arabidopsis thaliana culminates in testa rupture, which is followed by the late germination phase and endosperm rupture. An integrated view on the early phase of seed germination is provided and it is shown that it is characterized by dynamic biomechanical changes together with very early alterations in transcript, protein, and hormone levels that set the stage for the later events. Early seed germination thereby contributes to seed and seedling performance important for plant establishment in the natural and agricultural ecosystem.

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Plant Lipoxygenase: Structure and Function

J N Siedow (1991)

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Author and article information

Contributors

Dinakara Rao Ampasala: +91 413 2654946 , ampasaladr@bicpu.edu.in

Journal

Journal ID (nlm-ta): 3 Biotech

Journal ID (iso-abbrev): 3 Biotech

Title: 3 Biotech

Publisher: Springer Berlin Heidelberg (Berlin/Heidelberg )

ISSN (Print): 2190-572X

ISSN (Electronic): 2190-5738

Publication date (Electronic): 17 May 2016

Publication date PMC-release: 17 May 2016

Publication date (Print): June 2016

Volume: 6

Issue: 1

Electronic Location Identifier: 113

Affiliations

[1 ]Department of Biochemistry, Sri Venkateswara University, Tirupati, 517 502 Andhra Pradesh India

[2 ]Centre for Bioinformatics, School of Life sciences, Pondicherry University, Puducherry, 605014 India

Article

Publisher ID: 427

DOI: 10.1007/s13205-016-0427-5

PMC ID: 5398193

PubMed ID: 28330183

SO-VID: c8319846-95f8-4c09-baed-4f6f733b5d89

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

History

Date received : 7 November 2015

Date accepted : 26 April 2016

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001407, Department of Biotechnology , Ministry of Science and Technology;

Award ID: No: BT/PR10858/AGR/02/682/2008

Award Recipient : Dinakara Rao Ampasala

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Keywords: chromatofocusing,circular dichroism,mung bean,plant lipoxygenases,protein purification

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Keywords: chromatofocusing, circular dichroism, mung bean, plant lipoxygenases, protein purification

Purification and characterization of lipoxygenase from mung bean ( Vigna radiata L.) germinating seedlings

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Most cited references 29

The lipoxygenase pathway.

First off the mark: early seed germination.

Plant Lipoxygenase: Structure and Function

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