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      Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus

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          Abstract

          Background

          Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field.

          Results

          This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during the dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect the ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT, and GSH than the control group EC. The EC mainly depended on the AsA-GSH and GPX cycle to scavenge H 2O 2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H 2O 2 and MDA. The elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation.

          Conclusions

          The SWCNTs regulated oxidative stress responses of EC during the process and controlled oxidative damages by the maintenance of ROS homeostasis to achieve a high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology.

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          Plant nanobionics approach to augment photosynthesis and biochemical sensing.

          Juan Giraldo, Markita P. Landry, Sean M. Faltermeier … (2014)
          The interface between plant organelles and non-biological nanostructures has the potential to impart organelles with new and enhanced functions. Here, we show that single-walled carbon nanotubes (SWNTs) passively transport and irreversibly localize within the lipid envelope of extracted plant chloroplasts, promote over three times higher photosynthetic activity than that of controls, and enhance maximum electron transport rates. The SWNT-chloroplast assemblies also enable higher rates of leaf electron transport in vivo through a mechanism consistent with augmented photoabsorption. Concentrations of reactive oxygen species inside extracted chloroplasts are significantly suppressed by delivering poly(acrylic acid)-nanoceria or SWNT-nanoceria complexes. Moreover, we show that SWNTs enable near-infrared fluorescence monitoring of nitric oxide both ex vivo and in vivo, thus demonstrating that a plant can be augmented to function as a photonic chemical sensor. Nanobionics engineering of plant function may contribute to the development of biomimetic materials for light-harvesting and biochemical detection with regenerative properties and enhanced efficiency.
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            Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants.

            Y Kovtun, W Chiu, G. Tena … (2000)
            Despite the recognition of H(2)O(2) as a central signaling molecule in stress and wounding responses, pathogen defense, and regulation of cell cycle and cell death, little is known about how the H(2)O(2) signal is perceived and transduced in plant cells. We report here that H(2)O(2) is a potent activator of mitogen-activated protein kinases (MAPKs) in Arabidopsis leaf cells. Using epitope tagging and a protoplast transient expression assay, we show that H(2)O(2) can activate a specific Arabidopsis mitogen-activated protein kinase kinase kinase, ANP1, which initiates a phosphorylation cascade involving two stress MAPKs, AtMPK3 and AtMPK6. Constitutively active ANP1 mimics the H(2)O(2) effect and initiates the MAPK cascade that induces specific stress-responsive genes, but it blocks the action of auxin, a plant mitogen and growth hormone. The latter observation provides a molecular link between oxidative stress and auxin signal transduction. Finally, we show that transgenic tobacco plants that express a constitutively active tobacco ANP1 orthologue, NPK1, display enhanced tolerance to multiple environmental stress conditions without activating previously described drought, cold, and abscisic acid signaling pathways. Thus, manipulation of key regulators of an oxidative stress signaling pathway, such as ANP1/NPK1, provides a strategy for engineering multiple stress tolerance that may greatly benefit agriculture.
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              Abiotic stress signalling pathways: specificity and cross-talk.

              Heather Knight, Marc R. Knight (2001)
              Plants exhibit a variety of responses to abiotic stresses that enable them to tolerate and survive adverse conditions. As we learn more about the signalling pathways leading to these responses, it is becoming clear that they constitute a network that is interconnected at many levels. In this article, we discuss the 'cross-talk' between different signalling pathways and question whether there are any truly specific abiotic stress signalling responses.
              Article 0 comments Cited 199 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Hairong Chen:
                ORCID: http://orcid.org/0000-0003-3996-5857
                saasdus@163.com
                Di Zhang: zhangdi2013@sjtu.edu.cn
                Journal
                Journal ID (nlm-ta): Plant Methods
                Journal ID (iso-abbrev): Plant Methods
                Title: Plant Methods
                Publisher: BioMed Central (London )
                ISSN (Electronic): 1746-4811
                Publication date (Electronic): 21 September 2020
                Publication date PMC-release: 21 September 2020
                Publication date Collection: 2020
                Volume: 16
                Electronic Location Identifier: 130
                Affiliations
                [1 ]GRID grid.419073.8, ISNI 0000 0004 0644 5721, Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, ; NO. 888, Rd. Yezhuang, Shanghai, 201403 China
                [2 ]GRID grid.16821.3c, ISNI 0000 0004 0368 8293, Department of Landscape Science and Engineering, School of Design, , Shanghai Jiao Tong University, ; NO. 800, Rd. Dong Chuan, Shanghai, 200240 China
                Author information
                http://orcid.org/0000-0001-8255-7678
                http://orcid.org/0000-0003-3996-5857
                Article
                Publisher ID: 674
                DOI: 10.1186/s13007-020-00674-6
                PMC ID: 7507619
                PubMed ID: 32973916
                SO-VID: c662e363-8b17-4f5c-8fa4-6c6f3fda3f43
                Copyright © © The Author(s) 2020
                License:

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

                History
                Date received : 10 May 2020
                Date accepted : 15 September 2020
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;
                Award ID: 31870686
                Award ID: 31670693
                Award ID: 31971705
                Award Recipient :
                Funded by: Academic Specialty Development Project of Shanghai Academy of Agricultural Sciences
                Award ID: 2019-13
                Award Recipient :
                Categories
                Subject: Research
                Custom metadata
                issue-copyright-statement © The Author(s) 2020

                ScienceOpen disciplines: Plant science & Botany
                Keywords: agapanthus praecox,cryopreservation,oxidative stress,single-wall carbon nanotubes
                Data availability:
                ScienceOpen disciplines: Plant science & Botany
                Keywords: agapanthus praecox, cryopreservation, oxidative stress, single-wall carbon nanotubes

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