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      Crescimento e esporulação de isolados de Verticillium lecanii sob diferentes fatores ambientais Translated title: Growth and sporulation of Verticillium lecanii isolates under different environmental conditions

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          Abstract

          O objetivo deste trabalho foi avaliar o crescimento e a esporulação de dois isolados (JAB 02 e JAB 45) de Verticillium lecanii (Zimm.) Viégas, submetidos a diferentes valores de pH (4, 5, 6, 7, 8, 9) inicial do meio de cultivo sólido, de temperatura (19, 22, 25, 28 e 31ºC) e fotoperíodo (0, 12 e 24 horas). Após 20 dias de cultivo, não se observou efeito do pH inicial do meio no crescimento e esporulação dos isolados, exceto no pH 4,0 que reduziu o crescimento. A ausência de iluminação proporcionou a melhor condição para o crescimento de JAB 02 (32,70 mm) e JAB 45 (32,67 mm). Não houve efeito do fotoperíodo na produção de esporos. As melhores temperaturas para o crescimento de JAB 02 foram 19, 22 e 25ºC e para JAB 45 foram 19 e 25ºC. Este último isolado apresentou melhor esporulação a 19 e 22ºC, mas a temperatura não influenciou a esporulação de JAB 02. Não houve desenvolvimento dos isolados a 31ºC.

          Translated abstract

          This research aimed to evaluate the growth and sporulation of two Verticillium lecanii (Zimm.) Viégas isolates cultivated on solid medium with different initial pH values (4, 5, 6, 7, 8 and 9), exposed to different temperatures (19, 22, 25, 28 and 31ºC) and photoperiods (0, 12 and 24 hours). There was no substantial effect of the initial pH values on growth and sporulation of both isolates twenty days after inoculation, except at pH 4.0 in which the growth was significantly reduced. Absence of light promoted the best growth of JAB 02 (32.70 mm) and JAB 45 (32.67 mm) isolates but there was no effect of photoperiod on sporulation. The best temperatures for growth of JAB 02 were 19, 22 and 25ºC and for JAB 45, 19 and 25ºC. The latter isolate sporulated most at 19 and 22ºC and there was no effect of temperature on sporulation of JAB 02. None of the isolates developed at 31ºC.

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          Most cited references16

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          Controle microbiano de insetos

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            Culture Age, Temperature, and pH Affect the Polyol and Trehalose Contents of Fungal Propagules.

            The growth and conidial physiology of the entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces farinosus were studied under different conditions. The effects of culture age (up to 120 days), temperature (5 to 35(deg)C), and pH (2.9 to 11.1) were determined. Growth was optimal at pH 5 to 8 for each isolate and between 20 and 35(deg)C, depending on the isolate. The predominant polyol in conidia was mannitol, with up to 39, 134, and 61 mg g of conidia(sup-1) for B. bassiana, M. anisopliae, and P. farinosus, respectively. Conidia of M. anisopliae contained relatively small amounts of lower-molecular-weight polyols and trehalose (less than 25 mg g(sup-1) in total) in all treatments. Conidia of B. bassiana and P. farinosus contained up to 30, 32, and 25 mg of glycerol, erythritol, and trehalose, respectively, g(sup-1), depending on the treatment. Conidia of P. farinosus contained unusually high amounts of glycerol and erythritol at pH 2.9. The apparent effect of pH on gene expression is discussed in relation to the induction of a water stress response. To our knowledge, this is the first report of polyols and trehalose in fungal propagules produced over a range of temperature or pH. Some conditions and harvesting times were associated with an apparent inhibition of synthesis or accumulation of polyols and trehalose. This shows that culture age and environmental conditions affect the physiological quality of inoculum and can thereby determine its potential for biocontrol.
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              Physiology of fungi

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                Author and article information

                Journal
                pab
                Pesquisa Agropecuária Brasileira
                Pesq. agropec. bras.
                Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira (Brasília, DF, Brazil )
                0100-204X
                1678-3921
                June 2004
                : 39
                : 6
                : 561-565
                Affiliations
                [02] orgnameUniversidade Estadual Paulista (Unesp) orgdiv1Fac. de Ciências Agrárias e Veterinárias (FCAV) orgdiv2Dep. de Fitossanidade antoniac@ 123456fcav.unesp.br
                [03] orgnameUniversidade Estadual Paulista (Unesp) orgdiv1Fac. de Ciências Agrárias e Veterinárias (FCAV) orgdiv2Dep. de Ciências Exatas genertp@ 123456fcav.unesp.br
                [01] Jaboticabal SP orgnameUniversidade Estadual Paulista (Unesp) orgdiv1Fac. de Ciências Agrárias e Veterinárias (FCAV) orgdiv2Dep. de Produção Vegetal montecar@ 123456fcav.unesp.br
                Article
                S0100-204X2004000600007 S0100-204X(04)03900607
                c50822f7-9f33-4063-8c1f-30efc73d9326

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 29 March 2004
                : 21 November 2003
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 19, Pages: 5
                Product

                SciELO Brazil

                Categories
                Microbiologia

                pest control,photoperiod,temperature,pH,controle de pragas,fotoperíodo,temperatura,fungo entomopatogênico,entomopathogenic fungi

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