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      A lipidomic approach to identify cold-induced changes in Arabidopsis membrane lipid composition

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          Abstract

          Lipid changes that occur in leaves of Arabidopsis thaliana during cold and freezing stress of plants can be analyzed with electrospray ionization triple quadrupole mass spectrometry, using high-throughput multiple reaction monitoring (MRM). An online tool, LipidomeDB Data Calculation Environment, is employed for mass spectral data processing.

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          Author and article information

          Journal
          Journal ID (nlm-journal-id): 9214969
          Journal ID (pubmed-jr-id): 2488
          Journal ID (nlm-ta): Methods Mol Biol
          Journal ID (iso-abbrev): Methods Mol Biol
          Title: Methods in molecular biology (Clifton, N.J.)
          ISSN (Print): 1064-3745
          ISSN (Electronic): 1940-6029
          Publication date Nihms-submitted: 2 March 2021
          Publication date (Print): 2020
          Publication date PMC-release: 24 March 2021
          Volume: 2156
          Pages: 187-202
          Affiliations
          Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS 66506, USA
          Author notes
          [1]

          Current address: Children’s Medical Research Institute at University of Texas-Southwestern, 6000 Harry Hines Blvd., Dallas, TX 75235

          [2]

          Current address: Department of Biochemistry and Molecular Biophysics, Kansas State University, Chalmers Hall, 1711 Claflin Rd., Manhattan, KS 66506

          [* ]Corresponding author: Kansas Lipidomics Research Center, Division of Biology, Ackert Hall, Kansas State University, 1717 Claflin Rd., Manhattan, KS 66506, USA, Phone: 785-532-6241, welti@ 123456ksu.edu
          Article
          Accession ID: PMC7988500 Pmcid ID: PMC7988500 Pmc-uid ID: 7988500 Manuscript ID: nihpa1676880
          DOI: 10.1007/978-1-0716-0660-5_14
          PMC ID: 7988500
          PubMed ID: 32607983
          SO-VID: c4c5c826-6be0-4f1f-9a58-7ab58ec090dd
          History
          Categories
          Subject: Article

          Keywords: Mass spectrometry,Cold,Freezing,Multiple reaction monitoring (MRM),Lipidomics,Lipidomics data processing,Post-freezing recovery
          Data availability:
          Keywords: Mass spectrometry, Cold, Freezing, Multiple reaction monitoring (MRM), Lipidomics, Lipidomics data processing, Post-freezing recovery

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