9
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck. LSCC patients have seriously impaired vocal, respiratory, and swallowing functions with poor prognosis. Circular RNA (circRNA) has attracted great attention in cancer research. However, the expression patterns and roles of circRNAs in LSCC remain largely unknown.

          Methods

          RNA sequencing was performed on 57 pairs of LSCC and matched adjacent normal mucosa tissues to construct circRNA, miRNA, and mRNA expression profiles. RT-PCR, qPCR, Sanger sequencing, and FISH were undertaken to study the expression, localization, and clinical significance of circCORO1C in LSCC tissues and cells. The functions of circCORO1C in LSCC were investigated by RNAi-mediated knockdown, proliferation analysis, EdU staining, colony formation assay, Transwell assay, and apoptosis analysis. The regulatory mechanisms among circCORO1C, let-7c-5p, and PBX3 were investigated by luciferase assay, RNA immunoprecipitation, western blotting, and immunohistochemistry.

          Results

          circCORO1C was highly expressed in LSCC tissues and cells, and this high expression was closely associated with the malignant progression and poor prognosis of LSCC. Knockdown of circCORO1C inhibited the proliferation, migration, invasion, and in vivo tumorigenesis of LSCC cells. Mechanistic studies revealed that circCORO1C competitively bound to let-7c-5p and prevented it from decreasing the level of PBX3, which promoted the epithelial–mesenchymal transition and finally facilitated the malignant progression of LSCC.

          Conclusions

          circCORO1C has an oncogenic role in LSCC progression and may serve as a novel target for LSCC therapy. circCORO1C expression has the potential to serve as a novel diagnostic and prognostic biomarker for LSCC detection.

          Related collections

          Most cited references23

          • Record: found
          • Abstract: found
          • Article: found
          Is Open Access

          The circRNA circAGFG1 acts as a sponge of miR-195-5p to promote triple-negative breast cancer progression through regulating CCNE1 expression

          Background In recent years, circular RNAs (circRNAs), a new star of non-coding RNA, have been emerged as vital regulators and gained much attention for involvement of initiation and progression of diverse kinds of human diseases, especially cancer. However, regulatory role, clinical significance and underlying mechanisms of circRNAs in triple-negative breast cancer (TNBC) still remain largely unknown. Methods Here, the expression profile of circRNAs in 4 pairs of TNBC tissues and adjacent non-tumor tissues was analyzed by RNA-sequencing. Quantitative real-time PCR and in situ hybridization were used to determine the level and prognostic values of circAGFG1 in two TNBC cohorts. Then, functional experiments in vitro and in vivo were performed to investigate the effects of circAGFG1 on tumor growth and metastasis in TNBC. Mechanistically, fluorescent in situ hybridization, dual luciferase reporter assay, RNA pull-down and RNA immunoprecipitation experiments were performed to confirm the interaction between circAGFG1 and miR-195-5p in TNBC. Results We found that circAGFG1 was evidently up-regulated in TNBC, and its level was correlated with clinical stage, pathological grade and poor prognosis of patients with TNBC. The results indicated that circAGFG1 could promote TNBC cell proliferation, mobility and invasion as well as tumorigenesis and metastasis in vivo. Mechanistic analysis showed that circAGFG1 may act as a ceRNA (competing endogenous RNA) of miR-195-5p to relieve the repressive effect of miR-195-5p on its target cyclin E1 (CCNE1). Conclusions Our findings suggest that circAGFG1 promotes TNBC progression through circAGFG1/miR-195-5p/CCNE1 axis and it may serve as a new diagnostic marker or target for treatment of TNBC patients. Electronic supplementary material The online version of this article (10.1186/s12943-018-0933-7) contains supplementary material, which is available to authorized users.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: found
            Is Open Access

            Circular RNAs: Biogenesis, Mechanism, and Function in Human Cancers

            CircRNAs are a class of noncoding RNA species with a circular configuration that is formed by either typical spliceosome-mediated or lariat-type splicing. The expression of circRNAs is usually abnormal in many cancers. Several circRNAs have been demonstrated to play important roles in carcinogenesis. In this review, we will first provide an introduction of circRNAs biogenesis, especially the regulation of circRNA by RNA-binding proteins, then we will focus on the recent findings of circRNA molecular mechanisms and functions in cancer development. Finally, some open questions are also discussed.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              MicroRNA let-7c inhibits migration and invasion of human non-small cell lung cancer by targeting ITGB3 and MAP4K3.

              MicroRNAs play an important regulatory role in carcinogenesis and cancer metastasis. Different members of let-7 family have been reported to be decreased in human lung tumors. However, the effect of specific let-7 member on metastasis of NSCLC remains undefined. Our current study detected the expression of let-7 members in 94 cases of NSCLC and a significant association was noticed between low levels of let-7c expression and metastasis, venous invasion, advanced TNM stages and poor survival of NSCLC patients. Consistently, ectopic expression of let-7c in relatively highly metastatic cells remarkably suppressed their migration and invasion. Inhibition of let-7c in cells with relatively low metastatic potential promoted their motility and invasion. We then analyzed the potential targets of let-7c and found that ITGB3 and MAP4K3 were directly repressed by let-7c. Upon restoring the expression of ITGB3 and MAP4K3, the effects of let-7c on tumor metastasis were partially reversed, and more importantly, the expression levels of ITGB3 and MAP4K3 were inversely correlated with let-7c in 64 NSCLC tissues. Collectively, our results suggest that let-7c, by degrading ITGB3 and MAP4K3, prevents NSCLC metastasis. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.
                Bookmark

                Author and article information

                Contributors
                anchangming@cicams.ac.cn
                huizhengli2004@163.com
                xuwhns@126.com
                gaoweisxent@sxent.org
                Journal
                Mol Cancer
                Mol. Cancer
                Molecular Cancer
                BioMed Central (London )
                1476-4598
                2 June 2020
                2 June 2020
                2020
                : 19
                : 99
                Affiliations
                [1 ]GRID grid.263452.4, ISNI 0000 0004 1798 4018, Shanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, , Shanxi Medical University, ; Taiyuan, 030001 Shanxi People’s Republic of China
                [2 ]GRID grid.452461.0, ISNI 0000 0004 1762 8478, Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, , The First Hospital of Shanxi Medical University, ; Taiyuan, 030001 Shanxi People’s Republic of China
                [3 ]GRID grid.452461.0, ISNI 0000 0004 1762 8478, Department of Otolaryngology Head & Neck Surgery, , The First Hospital of Shanxi Medical University, ; Taiyuan, 030001 Shanxi People’s Republic of China
                [4 ]GRID grid.263452.4, ISNI 0000 0004 1798 4018, Key Laboratory of Cellular Physiology, Ministry of Education, , Shanxi Medical University, ; Taiyuan, 030001 Shanxi People’s Republic of China
                [5 ]GRID grid.263452.4, ISNI 0000 0004 1798 4018, Department of Biochemistry & Molecular Biology, , Shanxi Medical University, ; Taiyuan, 030001 Shanxi People’s Republic of China
                [6 ]GRID grid.454145.5, ISNI 0000 0000 9860 0426, Department of Otolaryngology Head & Neck Surgery, , The First Hospital, Jinzhou Medical University, ; Jinzhou, 121001 Liaoning People’s Republic of China
                [7 ]GRID grid.263452.4, ISNI 0000 0004 1798 4018, Department of Pathology, Shanxi Cancer Hospital, , Shanxi Medical University, ; Taiyuan, 030013 Shanxi People’s Republic of China
                [8 ]GRID grid.254020.1, ISNI 0000 0004 1798 4253, Department of Otolaryngology Head & Neck Surgery, , Heping Hospital Affiliated to Changzhi Medical College, ; Changzhi, 046000 Shanxi People’s Republic of China
                [9 ]GRID grid.263452.4, ISNI 0000 0004 1798 4018, Department of Cell Biology and Genetics, , Basic Medical School of Shanxi Medical University, ; Taiyuan, 030001 Shanxi People’s Republic of China
                [10 ]GRID grid.263452.4, ISNI 0000 0004 1798 4018, Department of Physiology, , Shanxi Medical University, ; Taiyuan, 030001 Shanxi People’s Republic of China
                [11 ]GRID grid.13402.34, ISNI 0000 0004 1759 700X, Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, , Zhejiang University School of Medicine, ; Hangzhou 310009 Zhejiang, People’s Republic of China
                [12 ]GRID grid.1012.2, ISNI 0000 0004 1936 7910, Harry Perkins Institute of Medical Research, QEII Medical Centre and Centre for Medical Research, , the University of Western Australia, ; PO Box 7214, 6 Verdun Street, Nedlands, Perth, Western Australia 6009 Australia
                [13 ]GRID grid.263488.3, ISNI 0000 0001 0472 9649, General Hospital, , Shenzhen University, ; Shenzhen, 518055 Guangdong People’s Republic of China
                [14 ]GRID grid.506261.6, ISNI 0000 0001 0706 7839, Department of Head and Neck Surgery, Cancer Hospital, National Cancer Center, , Chinese Academy of Medical Sciences & Peking Union Medical College, ; Beijing, 100021 People’s Republic of China
                [15 ]GRID grid.411971.b, ISNI 0000 0000 9558 1426, Department of Otolaryngology Head & Neck Surgery, Dalian Municipal Friendship Hospital, , Dalian Medical University, ; Dalian, 116100 Liaoning People’s Republic of China
                [16 ]GRID grid.27255.37, ISNI 0000 0004 1761 1174, Shandong Provincial ENT Hospital Affiliated to Shandong University, ; Jinan, 250022 Shandong People’s Republic of China
                [17 ]Shandong Provincial Institute of Otolaryngology, Jinan, 250022 Shandong People’s Republic of China
                [18 ]GRID grid.27255.37, ISNI 0000 0004 1761 1174, Key Laboratory of Otolaryngology, Ministry of Health, , Shandong University, ; Jinan, 250022 Shandong People’s Republic of China
                Author information
                http://orcid.org/0000-0001-7836-2851
                Article
                1215
                10.1186/s12943-020-01215-4
                7265647
                32487167
                c29d0453-1903-4645-bf2d-6dc9988e7b4c
                © The Author(s) 2020

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

                History
                : 20 January 2020
                : 11 May 2020
                Funding
                Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;
                Award ID: 81872210
                Award ID: 81802793
                Award ID: 81802948
                Award Recipient :
                Funded by: FundRef http://dx.doi.org/10.13039/501100010031, Postdoctoral Research Foundation of China;
                Award ID: 2016M591412
                Award ID: 2017M610174
                Award Recipient :
                Funded by: The Excellent talent science and technology innovation project of Shanxi Province
                Award ID: 201605D211029
                Award ID: 201705D211018
                Award ID: 201805D211007
                Award Recipient :
                Funded by: Youth Science and Technology Research Fund of Shanxi Province
                Award ID: 201901D211486
                Award ID: 201901D211490
                Award Recipient :
                Funded by: Shanxi Province Scientific and Technological Achievements Transformation Guidance Foundation
                Award ID: 201604D131002
                Award ID: 201604D132040
                Award ID: 201804D131043
                Award Recipient :
                Funded by: Youth Foundation of The First Hospital Affiliated with Shanxi Medical University
                Award ID: YQ1503
                Award Recipient :
                Funded by: Youth Top Talent Program Fund of Shanxi Province
                Award ID: 20180610
                Award Recipient :
                Funded by: Fund of Shanxi “1331” Project
                Categories
                Research
                Custom metadata
                © The Author(s) 2020

                Oncology & Radiotherapy
                circcoro1c,let-7c-5p,pbx3,laryngeal squamous cell carcinoma,epithelial–mesenchymal transition,metastasis

                Comments

                Comment on this article