For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
5
views
24
references
 Top references
cited by
3
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      451
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

      research-article

      Read this article at

      ScienceOpenPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background and Objectives:

          Acinetobacter baumannii has been known as a major pathogen causing nosocomial infections. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical isolates of A. baumannii.

          Materials and Methods:

          In this study, we used three sets of primers to amplify the MBL genes including bla OXA-48 , bla OXA-23 and bla NDM . The multiplex PCR assay was optimized for rapid and simultaneous detection of MBL genes in A. baumannii strains recovered from clinical samples.

          Results:

          A. baumannii strains recovered from clinical samples were subjected to the study. The multiplex PCR produced 3 bands of 501 bp for bla OXA-23 , 744 bp for bla OXA-48 and 623 bp for bla NDM genes. In addition to, no any cross-reactivity was observed in multiplex PCR.

          Conclusion:

          Based on obtained data, the multiplex PCR had a good specificity without any cross reactivity and it appears that the multiplex PCR is reliable assay for simultaneous detection of MBL genes in A. baumannii strains.

          Related collections

          Most cited references24

          • Record: found
          • Abstract: found
          • Article: not found

          How to detect NDM-1 producers.

          R. Walsh, Mark Toleman, Patrice Nordmann … (2011)
          Enterobacterial isolates expressing the carbapenemase NDM-1 are emerging worldwide. Twenty-seven NDM-1-positive isolates of worldwide origin were included in this study to identify these strains as not only pathogens but also colonizers of normal flora for infection control screening. Although susceptibility to carbapenems varied, a combined test (IMP/IMP + EDTA), the Etest MBL, and automated susceptibility testing by Vitek2 (bioMérieux) identified those NDM-1 producers as verified by PCR using specific primers. Screening for carriers of NDM-1 producers may be based on media such as the ChromID ESBL culture medium routinely used to screen for extended-spectrum β-lactamase producers, which gives excellent detection levels with low limits of detection ranging from 8 × 10(0) to 5 × 10(2) CFU/ml. The CHROMagar KPC culture medium had higher limits of detection (1 × 10(1) to 5 × 10(5) CFU/ml) and may be proposed for the follow-up of outbreaks of infections with NDM-1 producers. Colonies growing on these screening media can be verified as NDM-1 producers with molecular methods as described herein.
          Article 0 comments Cited 106 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Insights into the global molecular epidemiology of carbapenem non-susceptible clones of Acinetobacter baumannii.

            Nabil Karah, Arnfinn Sundsfjord, Kevin Towner … (2012)
            The global emergence of multidrug resistance (MDR) among Gram-negative bacteria has dramatically limited the therapeutic options. During the last two decades, Acinetobacter baumannii has become a pathogen of increased clinical importance due to its remarkable ability to cause outbreaks of infections and to acquire resistance to almost all currently used antibiotics, including the carbapenems. This review considers the literature on A. baumannii and data from multilocus sequence typing studies to explore the global population structure of A. baumannii and detect the occurrence of clonality, with the focus on the presence of specific resistance mechanisms such as the OXA-carbapenemases. The worldwide dissemination of MDR and carbapenem non-susceptible A. baumannii is associated with diverse genetic backgrounds, but predominated by a number of extensively distributed clones, such as CC92(B)/CC2(P) and CC109(B)/CC1(P), which have frequently been supplemented by acquired OXA-type carbapenemase genes. Copyright © 2012 Elsevier Ltd. All rights reserved.
            Article 0 comments Cited 106 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Multidrug-resistant Acinetobacter baumannii: mechanisms of virulence and resistance.

              Nicola C. Gordon, David W. Wareham (2010)
              Infection due to Acinetobacter baumannii has become a significant challenge to modern healthcare systems. The organism shows a formidable capacity to develop antimicrobial resistance, yet the clinical impact of A. baumannii infection remains unclear. Much is known about the processes involved in multidrug resistance, but those underlying the pathogenicity and virulence potential of the organism are only beginning to be elucidated. In this article, we provide an overview of current knowledge, focusing on mechanisms of pathogenesis, the molecular basis of resistance and options for treatment in the absence of novel therapeutic agents. Copyright 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
              Article 0 comments Cited 100 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Iran J Microbiol
                Journal ID (iso-abbrev): Iran J Microbiol
                Journal ID (publisher-id): IJM
                Journal ID (pmc): IJM
                Title: Iranian Journal of Microbiology
                Publisher: Tehran University of Medical Sciences
                ISSN (Print): 2008-3289
                ISSN (Electronic): 2008-4447
                Publication date (Print): April 2020
                Volume: 12
                Issue: 2
                Pages: 107-112
                Affiliations
                Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
                Author notes
                [* ]Corresponding author: Reza Ranjbar, PhD, Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran., Tel: +98-21-88039883, Fax: +98-21-88039883, Email: ranjbarre@ 123456gmail.com
                Article
                Publisher ID: IJM-12-107
                PMC ID: 7244819
                PubMed ID: 32494344
                SO-VID: c24985f4-3bf0-4112-8817-276f00c6aad7
                Copyright © Copyright© 2020 Iranian Neuroscience Society
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : February 2019
                Date accepted : February 2020
                Categories
                Subject: Original Article

                ScienceOpen disciplines: Microbiology & Virology
                Keywords: acinetobacter baumannii,metallo-β-lactamase,multiplex polymerase chain reaction
                Data availability:
                ScienceOpen disciplines: Microbiology & Virology
                Keywords: acinetobacter baumannii, metallo-β-lactamase, multiplex polymerase chain reaction

                Comments

                Comment on this article

                Similar content451

                See all similar

                Cited by3

                See all cited by

                Most referenced authors257

                See all reference authors