Effect of the Addition of Humic Substances as Growth Promoter in Broiler Chickens Under Two Feeding Regimens

Abstract

Simple Summary

The rapid spread of antimicrobial-resistant genes in bacterial communities is a threat to human, animal, and environmental health that continues to progress inflexibly. Humic substances (HS) are promising complex molecules as an alternative to reduce the use of growth promoter antibiotics (GPA) in animal feeds. Improvements in productivity, intestinal health, immune response, and antioxidant status have been reported in broilers supplemented with HS. In the present study, broilers fed with an extract of HS (EHS) from a worm compost had similar carcass yield and excretion of coccidian oocysts but increased Clostridium perfringens and lactic acid bacteria (LAB) compared to broilers fed diets added with GPA. Broilers subjected to feed restriction had reduced growth performance and meat quality. These results confirm the growth-promoting effect of ESH, which could be explained by higher abundance of the beneficial LAB and by reducing the multiplication of harmful parasites in the gut of broilers.

Abstract

Humic substances (HS) from different sources have been evaluated to replace or reduce the use of growth promoter antibiotics (GPA) in the feeds of broiler chickens. The objective was to evaluate the growth performance, tibia measurements, nutrient balance, meat quality, and microbiological status of broiler fed with an HS extract (EHS) under ad libitum (ADLIB) or feed restriction (REST). Individually caged broilers ( n = 180, 14–35 day of age) were assigned to a factorial arrangement of three dietary treatments: (1) positive control with bacitracin methylene disalicylate (BMD) and salinomycin; (2) negative control without BMD nor salinomycin, and (3) same as negative control with 0.25% EHS, and two feeding regimens 1) ADLIB or REST for 24 h on d 1, 7, and 14. Results were subjected to ANOVA. Positive control and EHS-fed broilers showed higher carcass yield ( p < 0.05) and lower oocyst excretion ( p < 0.01) compared to negative control birds. Lactic acid bacteria (LAB) and Clostridium perfringens ( C. perfringens) were higher in negative control and EHS-broilers compared to positive control ( p < 0.01). In conclusion, higher carcass yield, lower C. perfringens and oocyst excretion were found in positive control and higher carcass yield, higher LAB and lower oocyst excretion were found in EHS-fed broilers. Broilers subjected to REST had reduced growth performance and meat quality. In conclusion, EHS could be used to increase the carcass yield and beneficial LAB in broilers.