The metabolic impact of extracellular nitrite on aerobic metabolism of Paracoccus denitrificans

Abstract

Nitrite, in equilibrium with free nitrous acid (FNA), can inhibit both aerobic and anaerobic growth of microbial communities through bactericidal activities that have considerable potential for control of microbial growth in a range of water systems. There has been much focus on the effect of nitrite/FNA on anaerobic metabolism and so, to enhance understanding of the metabolic impact of nitrite/FNA on aerobic metabolism, a study was undertaken with a model denitrifying bacterium Paracoccus denitrificans PD1222. Extracellular nitrite inhibits aerobic growth of P. denitrificans in a pH-dependent manner that is likely to be a result of both nitrite and free nitrous acid (p K _a  = 3.25) and subsequent reactive nitrogen oxides generated from the intracellular passage of FNA into P. denitrificans. Increased expression of a gene encoding a flavohemoglobin protein (Fhp) (Pden_1689) was observed in response to extracellular nitrite. Construction and analysis of a deletion mutant established Fhp to be involved in endowing nitrite/FNA resistance at high extracellular nitrite concentrations. Global transcriptional analysis confirmed nitrite-dependent expression of fhp and indicated that P. denitrificans expressed a number of stress response systems associated with protein, DNA and lipid repair. It is therefore suggested that nitrite causes a pH-dependent stress response that is due to the production of associated reactive nitrogen species, such as nitric oxide from the internalisation of FNA.

Graphical abstract

Highlights

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  Paracoccus denitrificans was studied to assess the impact of nitrite on aerobic metabolism.

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  Extracellular nitrite inhibits aerobic growth of P. denitrificans in a pH-dependent manner.

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  Increased expression of a flavohemoglobin was observed in response to extracellular nitrite.

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  Analysis of a mutant established flavohemoglobin to be involved in nitrite tolerance.

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  Only ∼ 1.5% of the genome was differentially regulated in response to nitrite ‘stress’.