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      Integration of spectral coronagraphy within VIPA-based spectrometers for high extinction Brillouin imaging

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      Optics Express
      The Optical Society

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          Abstract

          <p class="first" id="d15140413e165">VIPA (virtually imaged phase array) spectrometers have enabled rapid Brillouin spectrum measurements and current designs of multi-stage VIPA spectrometers offer enough spectral extinction to probe transparent tissue, cells and biomaterials. However, in highly scattering media or in the presence of strong back-reflections, such as at interfaces between materials of different refractive indices, VIPA-based Brillouin spectral measurements are limited. While several approaches to address this issue have recently been pursued, important challenges remain. Here we have adapted the design of coronagraphs used for exosolar planet imaging to the spectral domain and integrated it in a double-stage VIPA spectrometer. We demonstrate that this yields an increase in extinction up to 20 dB, with nearly no added insertion loss. The power of this improvement is vividly demonstrated by Brillouin imaging close to reflecting interfaces without index matching or sample tilting. </p>

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          Most cited references30

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          Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy

          Current measurements of the biomechanical properties of cells require physical contact with cells or lack sub-cellular resolution. Here, we developed a label-free optical microscopy technique based on Brillouin light scattering capable of measuring intracellular longitudinal modulus with optical resolution. We obtained 3D Brillouin maps of cells in 2D and 3D microenvironments, which reveal mechanical changes due to cytoskeletal modulation and cell volume regulation.
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            Confocal Brillouin microscopy for three-dimensional mechanical imaging.

            Acoustically induced inelastic light scattering, first reported in 1922 by Brillouin1, allows non-contact, direct readout of the viscoelastic properties of a material and has widely been investigated for material characterization2, structural monitoring3 and environmental sensing4. Extending the Brillouin technique from point sampling spectroscopy to imaging modality5 would open up new possibilities for mechanical imaging, but has been challenging because rapid spectrum acquisition is required. Here, we demonstrate a confocal Brillouin microscope based on a fully parallel spectrometer-a virtually imaged phased array-that improves the detection efficiency by nearly 100-fold over previous approaches. Using the system, we show the first cross-sectional Brillouin imaging based on elastic properties as the contrast mechanism and monitor fast dynamic changes in elastic modulus during polymer crosslinking. Furthermore, we report the first in situ biomechanical measurement of the crystalline lens in a mouse eye. These results suggest multiple applications of Brillouin microscopy in biomedical and biomaterial science.
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              Brillouin optical microscopy for corneal biomechanics.

              The mechanical properties of corneal tissue are linked to prevalent ocular diseases and therapeutic procedures. Brillouin microscopy is a novel optical technology that enables three-dimensional mechanical imaging. In this study, the feasibility of this noncontact technique was tested for in situ quantitative assessment of the biomechanical properties of the cornea. Brillouin light-scattering involves a spectral shift proportional to the longitudinal modulus of elasticity of the tissue. A 532-nm single-frequency laser and a custom-developed ultrahigh-resolution spectrometer were used to measure the Brillouin frequency. Confocal scanning was used to perform Brillouin elasticity imaging of the corneas of whole bovine eyes. The longitudinal modulus of the bovine corneas was compared before and after riboflavin corneal collagen photo-cross-linking. The Brillouin measurements were then compared with conventional stress-strain mechanical test results. High-resolution Brillouin images of the cornea were obtained, revealing a striking depth-dependent variation of the elastic modulus across the cornea. Along the central axis, the Brillouin frequency shift varied gradually from 8.2 GHz in the epithelium to 7.5 GHz near the endothelium. The coefficients of the down slope were measured to be approximately 1.09, 0.32, and 2.94 GHz/mm in the anterior, posterior, and innermost stroma, respectively. On riboflavin collagen cross-linking, marked changes in the axial Brillouin profiles (P < 0.001) were noted before and after cross-linking. Brillouin imaging can assess the biomechanical properties of cornea in situ with high spatial resolution. This novel technique has the potential for use in clinical diagnostics and treatment monitoring.
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                Author and article information

                Journal
                OPEXFF
                Optics Express
                Opt. Express
                The Optical Society
                1094-4087
                2017
                2017
                March 15 2017
                March 20 2017
                : 25
                : 6
                : 6895
                Article
                10.1364/OE.25.006895
                5772426
                28381031
                bebaab51-9253-4372-b18f-6b3d2a17ea1d
                © 2017

                https://www.osapublishing.org/submit/licenses/license_v1.cfm#vor

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