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      Development and validation of an algorithm for laser application in wound treatment 1

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          ABSTRACT

          Objective:

          To develop and validate an algorithm for laser wound therapy.

          Method:

          Methodological study and literature review. For the development of the algorithm, a review was performed in the Health Sciences databases of the past ten years. The algorithm evaluation was performed by 24 participants, nurses, physiotherapists, and physicians. For data analysis, the Cronbach’s alpha coefficient and the chi-square test for independence was used. The level of significance of the statistical test was established at 5% (p<0.05).

          Results:

          The professionals’ responses regarding the facility to read the algorithm indicated: 41.70%, great; 41.70%, good; 16.70%, regular. With regard the algorithm being sufficient for supporting decisions related to wound evaluation and wound cleaning, 87.5% said yes to both questions. Regarding the participants’ opinion that the algorithm contained enough information to support their decision regarding the choice of laser parameters, 91.7% said yes. The questionnaire presented reliability using the Cronbach’s alpha coefficient test (α = 0.962).

          Conclusion:

          The developed and validated algorithm showed reliability for evaluation, wound cleaning, and use of laser therapy in wounds.

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          Most cited references47

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          Cicatrização: conceitos atuais e recursos auxiliares - Parte I

          - O estudo da cicatrização da pele envolve uma enorme gama de eventos e de situações especiais. Exige conhecimentos básicos de anatomia, histologia, bioquímica, imunologia, farmacologia, entre outras ciências. O artigo faz revisão dos conceitos de cicatrização, em seus diversos aspectos, e ressalta a importância da atuação multidisciplinar na abordagem das feridas, bem como a percepção do paciente como um todo. São abordados os aspectos econômicos que representam as feridas agudas e crônicas. Apresenta ainda os recursos que podem auxiliar o processo de cicatrização, bem como os diversos tipos de curativos disponíveis.
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            Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice.

            Low-level laser (light) therapy (LLLT) promotes wound healing, reduces pain and inflammation, and prevents tissue death. Studies have explored the effects of various radiant exposures on the effect of LLLT; however, studies of wavelength dependency in in vivo models are less common. In the present study, the healing effects of LLLT mediated by different wavelengths of light in the red and near-infrared (NIR) wavelength regions (635, 730, 810, and 980 nm) delivered at constant fluence (4 J/cm(2)) and fluence rate (10 mW/cm(2)) were evaluated in a mouse model of partial-thickness dermal abrasion. Wavelengths of 635 and 810 nm were found to be effective in promoting the healing of dermal abrasions. However, treatment using 730- and 980-nm wavelengths showed no sign of stimulated healing. Healing was maximally augmented in mice treated with an 810-nm wavelength, as evidenced by significant wound area reduction (p < 0.05), enhanced collagen accumulation, and complete re-epithelialization as compared to other wavelengths and non-illuminated controls. Significant acceleration of re-epithelialization and cellular proliferation revealed by immunofluorescence staining for cytokeratin-14 and proliferating cell nuclear antigen (p < 0.05) was evident in the 810-nm wavelength compared with other groups. Photobiomodulation mediated by red (635 nm) and NIR (810 nm) light suggests that the biological response of the wound tissue depends on the wavelength employed. The effectiveness of 810-nm wavelength agrees with previous publications and, together with the partial effectiveness of 635 nm and the ineffectiveness of 730 and 980 nm wavelengths, can be explained by the absorption spectrum of cytochrome c oxidase, the candidate mitochondrial chromophore in LLLT.
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              Content validity in the development and adaptation processes of measurement instruments.

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                Author and article information

                Journal
                Rev Lat Am Enfermagem
                Rev Lat Am Enfermagem
                rlae
                Revista Latino-Americana de Enfermagem
                Escola de Enfermagem de Ribeirão Preto / Universidade de São Paulo
                0104-1169
                1518-8345
                04 December 2017
                2017
                : 25
                : e2955
                Affiliations
                [2 ]MSc, Adjunct Professor, Phisiotherapy Department, Centro Universitário de Formiga , Formiga, MG, Brasil
                [3 ]PhD, Associate Professor, Universidade do Vale do Sapucaí, Pouso Alegre, MG, Brazil
                [4 ]MSc, Adjunct Professor, Phisiotherapy Department, Universidade do Vale do Sapucaí, Pouso Alegre, MG, Brazil
                [5 ]Pós-Doctoral degree, Full Professor, Plastic Surgery discipline, Universidade Federal de São Paulo, São Paulo, SP, Brazil
                Author notes
                Corresponding Author: Geraldo Magela Salomé Universidade do Vale do Sapucaí Faculdade de Ciências Médicas Dr. José Antônio Garcia Coutinho Av. Francisco de Paula Quintanilha Ribeiro, 280 Bairro: Jabaquara CEP: 04330-020, São Paulo, SP, Brasil E-mail: gsalome@ 123456infinitetrans.com
                Article
                00396
                10.1590/1518-8345.1998.2955
                5738875
                29211197
                bea3b095-8243-42e4-9fa2-02cb51c8e261
                Copyright © 2017 Revista Latino-Americana de Enfermagem

                This is an open-access article distributed under the terms of the Creative Commons Attribution License

                History
                : 18 December 2016
                : 27 August 2017
                Page count
                Figures: 6, Tables: 9, Equations: 0, References: 34, Pages: 1
                Categories
                Original Article

                wounds and injuries,wound healing,lasers,protocols,algorithms

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