Intravital microscopy datasets examining key nephron segments of transplanted decellularized kidneys

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

This study contains intravital microscopy (IVM) data examining the microarchitecture of acellular kidney scaffolds. Acellular scaffolds are cell-free collagen-based matrices derived from native organs that can be used as templates for regenerative medicine applications. This data set contains in vivo assays that evaluate the effectiveness of decellularization and how these acellular nephron compartments perform in the post-transplantation environment. Qualitative and quantitative assessments of scaffold DNA concentrations, tissue fluorescence signals, and structural and functional integrities of decellularized tubular and peritubular capillary segments were acquired and compared to the native (non-transplanted) organ. Cohorts of 2–3-month-old male Sprague Dawley rats were used: non-transplanted (n = 4), transplanted day 0 (n = 4), transplanted day 1 (n = 4), transplanted day 2 (n = 4), and transplanted day 7 (n = 4). Micrographs and supporting measurements are provided to illustrate IVM processes used to perform this study and are publicly available in a data repository to assist scientific reproducibility and extend the use of this powerful imaging application to analyze other scaffold systems.

Measurements(s)	DNA quantification • tissue fluorescence • microvascular leakage • tubular and peritubular capillary integrity
Technology Type(s)	intravital microscopy • multiphoton microscopy • UV-visible spectroscopy
Sample Characterization(s)	rats • native and decellularized kidneys

Abstract

Measurement(s)	DNA • tissue fluorescence
Technology Type(s)	ultraviolet-visible spectrophotometry • intravital microscopy
Sample Characteristic - Organism	mouse/rat NG108-15
Sample Characteristic - Environment	laboratory environment
Sample Characteristic - Location	contiguous United States of America • Emirate (United Arab Emirates)

Related collections

Most cited references 37

Record: found
Abstract: found
Article: found

Is Open Access

Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications

Anna Gilpin, Yong Yang (2017)

As the gap between donors and patients in need of an organ transplant continues to widen, research in regenerative medicine seeks to provide alternative strategies for treatment. One of the most promising techniques for tissue and organ regeneration is decellularization, in which the extracellular matrix (ECM) is isolated from its native cells and genetic material in order to produce a natural scaffold. The ECM, which ideally retains its inherent structural, biochemical, and biomechanical cues, can then be recellularized to produce a functional tissue or organ. While decellularization can be accomplished using chemical and enzymatic, physical, or combinative methods, each strategy has both benefits and drawbacks. The focus of this review is to compare the advantages and disadvantages of these methods in terms of their ability to retain desired ECM characteristics for particular tissues and organs. Additionally, a few applications of constructs engineered using decellularized cell sheets, tissues, and whole organs are discussed.

0 comments Cited 186 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering.

Yoshihide Hashimoto, Seiichi Funamoto, Shuji Sasaki … (2010)

To prepare acellular corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine corneas were hydrostatically pressed at 980 MPa at 10 or 30 degrees C for 10 min to make them opaque. There was no change in the thickness of the corneas immediately after the pressurization, but they swelled during the washing process. The cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the cornea decellularized with the HHP method, the complete removal of corneal cells was confirmed. Furthermore, when the corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native corneas. In an animal study, when acellular porcine corneas were implanted into rabbit cornea, no immune reaction occurred and the turbid corneas became clear. The decellularized corneas obtained through HHP could be useful as a corneal scaffold for tissue regeneration. Copyright 2010 Elsevier Ltd. All rights reserved.

0 comments Cited 74 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

In vivo multiphoton imaging of mitochondrial structure and function during acute kidney injury.

Bruce Molitoris, Nicholas Sandoval, Kenneth Rhodes … (2012)

Mitochondrial dysfunction has been implicated in the pathogenesis of acute kidney injury due to ischemia and toxic drugs. Methods for imaging mitochondrial function in cells using confocal microscopy are well established; more recently, it was shown that these techniques can be utilized in ex vivo kidney tissue using multiphoton microscopy. We extended this approach in vivo and found that kidney mitochondrial structure and function can be imaged in anesthetized rodents using multiphoton excitation of endogenous and exogenous fluorophores. Mitochondrial nicotinamide adenine dinucleotide increased markedly in rat kidneys in response to ischemia. Following intravenous injection, the mitochondrial membrane potential-dependent dye TMRM was taken up by proximal tubules; in response to ischemia, the membrane potential dissipated rapidly and mitochondria became shortened and fragmented in proximal tubules. In contrast, the mitochondrial membrane potential and structure were better maintained in distal tubules. Changes in mitochondrial structure, nicotinamide adenine dinucleotide, and membrane potential were found in the proximal, but not distal, tubules after gentamicin exposure. These changes were sporadic, highly variable among animals, and were preceded by changes in non-mitochondrial structures. Thus, real-time changes in mitochondrial structure and function can be imaged in rodent kidneys in vivo using multiphoton excitation of endogenous and exogenous fluorophores in response to ischemia-reperfusion injury or drug toxicity.

0 comments Cited 67 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Peter R. Corridon:

ORCID: http://orcid.org/0000-0002-6796-4301

peter.corridon@ku.ac.ae

Journal

Journal ID (nlm-ta): Sci Data

Journal ID (iso-abbrev): Sci Data

Title: Scientific Data

Publisher: Nature Publishing Group UK (London )

ISSN (Electronic): 2052-4463

Publication date (Electronic): 10 September 2022

Publication date PMC-release: 10 September 2022

Publication date Collection: 2022

Volume: 9

Electronic Location Identifier: 561

Affiliations

[1 ]GRID grid.440568.b, ISNI 0000 0004 1762 9729, Department of Immunology and Physiology, College of Medicine and Health Sciences, , Khalifa University of Science and Technology, ; PO Box 127788, Abu Dhabi, UAE

[2 ]GRID grid.412860.9, ISNI 0000 0004 0459 1231, Wake Forest Institute for Regenerative Medicine, , Medical Center Boulevard, ; Winston-Salem, NC 27157-1083 USA

[3 ]GRID grid.440568.b, ISNI 0000 0004 1762 9729, Biomedical Engineering, Healthcare Engineering Innovation Center, , Khalifa University of Science and Technology, ; PO Box 127788, Abu Dhabi, UAE

[4 ]GRID grid.440568.b, ISNI 0000 0004 1762 9729, Center for Biotechnology, , Khalifa University of Science and Technology, ; PO Box 127788, Abu Dhabi, UAE

Author information

Peter R. Corridon http://orcid.org/0000-0002-6796-4301

Article

Publisher ID: 1685

DOI: 10.1038/s41597-022-01685-9

PMC ID: 9464233

PubMed ID: 36088356

SO-VID: be3020e6-bbe3-4cdc-814c-f70746eff5b1

License:

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

History

Date received : 19 April 2022

Date accepted : 7 September 2022

Funding

Funded by: FundRef https://doi.org/10.13039/100000057, U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS);

Award ID: NIH/NIGMS K12-GM102773

Award Recipient : Peter R. Corridon

Funded by: FundRef https://doi.org/10.13039/501100004070, Khalifa University of Science, Technology and Research (Khalifa University);

Award ID: FSU-2020-25

Award ID: RC2-2018-022 (HEIC)

Award Recipient : Peter R. Corridon

Custom metadata

Keywords: end-stage renal disease,biological fluorescence,multiphoton microscopy

Data availability:

Keywords: end-stage renal disease, biological fluorescence, multiphoton microscopy

Intravital microscopy datasets examining key nephron segments of transplanted decellularized kidneys

Read this article at

Abstract

Abstract

Related collections

Atomic Force Microscopy

Most cited references 37

Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications

Preparation and characterization of decellularized cornea using high-hydrostatic pressurization for corneal tissue engineering.

In vivo multiphoton imaging of mitochondrial structure and function during acute kidney injury.

Author and article information

Contributors

Journal

Affiliations

Author information

Article

History

Funding

Categories

Custom metadata

Comments

Comment on this article

Similar content 649

Cited by 11

Most referenced authors 293