For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
18
views
34
references
 Top references
cited by
1
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      145
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      The Pavonia xanthogloea (Ekman, Malvaceae): Phenolic compounds quantification, anti-oxidant and cytotoxic effect on human lymphocytes cells

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Introduction:

          Pavonia xanthogloea is traditionally used as an antimicrobial and anti-tumour medicine in Southern Brazilian region. However, investigations about this species are still incipient.

          Hypothesis Tested:

          The study postulated that P. xanthologea specie present some phenolic compound and present some biological properties as anti-oxidant and cytoprotective effect against oxidative stress.

          Materials and Methods:

          The content of eight phenolic molecules in the crude ethanolic extract of the aerial part of P. xanthogloea and its five fractions (hexane, dichloromethane, ethyl-acetate, n-butanol, and water) was determined by heterotrophic plate count method. The anti-oxidant capacity of the extract and the fractions was determined by 1,1-diphenyl-2-picryl-hydrazyl assay. The potential anti-oxidant and cytoprotective effect was also analyzed in human lymphocyte culture treated with extract/fractions at different concentrations with and without oxidative stress generated by hydrogen peroxide (H 2O 2) and sodium nitroprusside (SNP) exposition.

          Results:

          Tiliroside was the molecule detected in all extract. Water and ethyl-acetate fractions showed the highest radical-scavenging activity. The crude extract, hexane, water, and n-butanol reversed the higher reactive oxygen specie levels generated by H 2O 2 and SNP to levels similar to those observed in the control group. In addition, crude extract, hexane, ethyl-acetate and n-butanol did not caused cytotoxicity, whereas water fraction was cytotoxic at higher concentration tested here (300 μg/mL). The cytotoxicity reversion caused by SNP exposition was concentration-dependent of the extract and fractions. However, dichloromethane fraction increased cell mortality in all concentrations investigated and was not able to decrease cell death in the lymphocytes exposed to SNP.

          Conclusion:

          The results suggest potential medicine use of this species.

          Related collections

          Most cited references34

          • Record: found
          • Abstract: found
          • Article: not found

          Bioactive bibenzyl derivatives and fluorenones from Dendrobium nobile.

          Xue Zhang, Jie-Kun Xu, Jue Wang … (2007)
          Bioassay-guided fractionation of the 60% ethanol extract of the stems of Dendrobium nobile using the DPPH assay led to the isolation of two new bibenzyl derivatives, nobilin D (1) and nobilin E (2), and a new fluorenone, nobilone (3), together with seven known compounds (4-10). Their structures were determined on the basis of spectroscopic analyses. Compounds 1, 2, 4, 7, 8, and 10 exhibited significant antioxidant activity higher than or equivalent to vitamin C in the DPPH assay, and compounds 1, 3, 4, and 7-10 displayed higher antioxidant activity than vitamin C in the ORAC assay. Compounds 1, 2, and 10 also exhibited stronger inhibitory effects on NO production than resveratrol.
          Article 0 comments Cited 42 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside.

            Araceli Sala, M.Carmen Recio, Guillermo Schinella … (2003)
            Three flavonoids, gnaphaliin, pinocembrin and tiliroside, isolated from Helichrysum italicum, were studied in vitro for their antioxidant and/or scavenger properties and in vivo in different models of inflammation. In vitro tests included lipid peroxidation in rat liver microsomes, superoxide radical generation in the xanthine/xanthine oxidase system and the reduction of the stable radical 1,1-diphenyl-2-pycryl-hydrazyl (DPPH). Acute inflammation was induced by application of 12-O-tetradecanoylphorbol 13-acetate (TPA) to the mouse ear or by subcutaneous injection of phospholipase A(2) or serotonin in the mouse paw. Eczema provoked on the mouse ear by repeated administration of TPA was selected as a model of chronic inflammation. The flavonoids were assayed against sheep red blood cell-induced mouse paw oedema as a model of delayed-type hypersensitivity reaction. The most active compound, both in vitro and in vivo, was tiliroside. It significantly inhibited enzymatic and non-enzymatic lipid peroxidation (IC(50)=12.6 and 28 microM, respectively). It had scavenger properties (IC(50)=21.3 microM) and very potent antioxidant activity in the DPPH test (IC(50)=6 microM). In vivo, tiliroside significantly inhibited the mouse paw oedema induced by phospholipase A(2)(ED(50)=35.6 mg/kg) and the mouse ear inflammation induced by TPA (ED(50)=357 microg/ear). Pinocembrin was the only flavonoid that exhibited anti-inflammatory activity in the sheep red blood cell-induced delayed-type hypersensitivity reaction. However, only tiliroside significantly reduced the oedema and leukocyte infiltration induced by TPA. As in the case of other flavonoids, the anti-inflammatory activity of tiliroside could be based on its antioxidant properties, although other mechanisms are probably involved.
            Article 0 comments Cited 39 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Anti-fungal and anti-bacterial activity of some herbal remedies from Tanzania.

              Anneleen Kool, William Mziray, Klaas van Gisbergen … (2005)
              Plants are not only important to the millions of people to whom traditional medicine serves as the only opportunity for health care and to those who use plants for various purposes in their daily lives, but also as a source of new pharmaceuticals. During interviews with the Pare people from Northeastern Tanzania, 29 plants that are used for medicinal purposes as well as 41 plants used for non-medicinal purposes were reported. Six medicinally used plants were selected for bioactivity analysis. Extracts of Coccinia adoensis, Cineraria grandiflora, Pavonia urens, Marattia fraxinea, Clutia abyssinica var. usambarica, and Vangueria infausta were made using ethyl acetate, methanol, cold water and boiling water. The antimicrobial activity was tested on Candida albicans, Aspergillus fumigatus, Fusarium culmorum, Staphylococcus aureus, Pseudomonas syringae, and Erwinia amylovora. All plants showed activity against several test organisms.
              Article 0 comments Cited 36 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Pharmacogn Mag
                Journal ID (iso-abbrev): Pharmacogn Mag
                Journal ID (publisher-id): PM
                Title: Pharmacognosy Magazine
                Publisher: Medknow Publications & Media Pvt Ltd (India )
                ISSN (Print): 0973-1296
                ISSN (Electronic): 0976-4062
                Publication date (Print): August 2014
                Volume: 10
                Issue: Suppl 3
                Pages: S630-S638
                Affiliations
                [1 ] Department of Physiology, Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, Brazil
                [2 ] Department of Chemistry, Federal University of Santa Maria, Santa Maria, Brazil
                [3 ] Department of Morphology, Laboratory of Biogenomics, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil
                [4 ] Department of Chemistry, Graduate Program in Biochemistry and Toxicology, Science Natural and Exact Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil
                Author notes
                Address for correspondence: Dr. Ivana Beatrice Manica da Cruz, Av Roraima 1000, Building 19, Laboratory of Biogenomic, Federal University of Santa Maria, Santa Maria-RS, Brazil – Zip Code 97105-900. E-mail: ibmcruz@ 123456hotmail.com
                Article
                Publisher ID: PM-10-630
                DOI: 10.4103/0973-1296.139804
                PMC ID: 4189282
                SO-VID: bd676c8a-733d-4e2c-bf92-bf768621c628
                Copyright © Copyright: © Pharmacognosy Magazine
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 18 September 2013
                Date revision received : 14 January 2014
                Date accepted : 30 August 2014
                Categories
                Subject: Original Article

                ScienceOpen disciplines: Pharmacology & Pharmaceutical medicine
                Keywords: anti-oxidant,cytotoxicity,oxidative stress,pavonia,phenolic compounds
                Data availability:
                ScienceOpen disciplines: Pharmacology & Pharmaceutical medicine
                Keywords: anti-oxidant, cytotoxicity, oxidative stress, pavonia, phenolic compounds

                Comments

                Comment on this article

                scite_

                Similar content145

                See all similar

                Cited by1

                See all cited by

                Most referenced authors217

                See all reference authors