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      Correction: Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS

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      The PLOS ONE Staff
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          Abstract

          Affiliation 4 and affiliation 5 are incorrectly switched. The affiliation for João Lopes should be: Departamento de Tecnologia Farmacêutica, Faculdade Farmácia, Universidade de Lisboa, Lisboa, Portugal. The affiliation for Manuela E. Pintado should be: CBQF, Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Porto, Portugal. The images for Figures 1 and 2 are incorrectly switched. The image that appears as Figure 1 should be Figure 2, and the image that appears as Figure 2 should be Figure 1. The figure legends appear in the correct order. Please view the correct images for Figure 1 and Figure 2 here. 10.1371/journal.pone.0110127.g001 Figure 1 Average mass spectra obtained by MALDI-TOF-MS analysis of B. pumilus and B. safensis isolates. (a) B. pumilus (5 spectra) and (b) B. safensis (22 spectra) in the ion range atm/z 2000 to 12000. Spectra were obtained by averaging the respective experimental ion signals from all isolates. 10.1371/journal.pone.0110127.g002 Figure 2 Score plot obtained by PLSDA regression model and the corresponding dendrogram. Score plot of the PLSDA regression model (a) and respective dendrogram (b), ofB. pumilus and B. safensis isolates. Legend: • B. pumilus isolates and ▾ B. safensis isolates. Unfilled symbols correspond to the type strains of both species.

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          Differentiation of Bacillus pumilus and Bacillus safensis Using MALDI-TOF-MS

          Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. Bacillus pumillus and Bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by conventional methodologies. In this study, using a well-characterized collection of B. pumillus and B. safensis isolates, we demonstrated the suitability of MALDI-TOF-MS combined with chemometrics to accurately and rapidly identify them. Moreover, characteristic species-specific ion masses were tentatively assigned, using UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases and primary literature. Delineation of B. pumilus (ions at m/z 5271 and 6122) and B. safensis (ions at m/z 5288, 5568 and 6413) species were supported by a congruent characteristic protein pattern. Moreover, using a chemometric approach, the score plot created by partial least square discriminant analysis (PLSDA) of mass spectra demonstrated the presence of two individualized clusters, each one enclosing isolates belonging to a species-specific spectral group. The generated pool of species-specific proteins comprised mostly ribosomal and SASPs proteins. Therefore, in B. pumilus the specific ion at m/z 5271 was associated with a small acid-soluble spore protein (SASP O) or with 50S protein L35, whereas in B. safensis specific ions at m/z 5288 and 5568 were associated with SASP J and P, respectively, and an ion at m/z 6413 with 50S protein L32. Thus, the resulting unique protein profile combined with chemometric analysis, proved to be valuable tools for B. pumilus and B. safensis discrimination, allowing their reliable, reproducible and rapid identification.
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            Author and article information

            Journal
            PLoS One
            PLoS ONE
            plos
            plosone
            PLoS ONE
            Public Library of Science (San Francisco, USA )
            1932-6203
            2014
            19 December 2014
            : 9
            : 12
            : e116426
            Article
            PONE-D-14-55435
            10.1371/journal.pone.0116426
            4272307
            25525812
            bc8e6651-319e-4658-a215-e0c05f3a3630
            Copyright @ 2014

            This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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